Putidaredoxin, the iron sulfur protein from the P450,,,mediated methylene hydroxylase system of Pseudomonas putida, is shown to be essential both as an electron transfer agent and as an effector of P450,,, necessary for product formation. P450,,, is recognized to progress through four well defined states in an ordered reaction cycle of oxidized, and, with substrate, oxidized, reduced, and oxyge...

in this research, we investigated the cytotoxic mechanisms of one of the widely used pharmaceuticals that are regularly associated with the adverse effects on the liver, sometimes leading to acute liver failure, diclofenac. diclofenac liver cytotoxicity was associated with reactive oxygen species (ros) formation and lipid peroxidation which were inhibited by antioxidants and ros scavengers, fer...

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Development and Validation of a New Simple Liquid Chromatographic Method for Determination Coumarin its 7-Hydroxy Metabolite as Marker Cytochrome P450 2A6 in RatsSundeep Bath, Wael Ebied, Hytham Ahmed, Fawzy Elbarbry

Abs t r a : The most fiequently encountered function of cytochromes P450, the monooxygenation of substrates, is now well understood and depends on the involvement of a high-valent iron-0x0 intermediate. The corresponding reactions have been reproduced by using metalloporphyrin model systems, the most efficient catalysts being Fe(III) or Mn(III) tetraarylporphyrins bearing electronwithdrawing su...

A sensing method based on resonance localized surface plasmon spectroscopy was developed for low molecular weight substrate and inhibitor molecules binding to heme proteins. Cytochrome P450 proteins have Soret and Q absorption bands in the visible wavelength region. The coupling between the molecular resonance of P450 and the localized surface plasmon resonance (LSPR) of functionalized silver n...

Nitric oxide (NO) modulates various metabolisms through interaction with thiol proteins and hemoproteins. Although NO interacts reversibly with iron moieties of heme proteins, including cytochrome P450 (P450), dynamic aspects of the formation, catalytic functions and fates of NO-P450 adducts remain to be elucidated. When incubated with NOC7, which spontaneously and stoichiometrically releases N...