A reverse phase high performance liquid chromatographic (RPHPLC) method has been developed and validated for simultaneous estimation of Naloxone Hydrochloride and Buprenorphine Hydrochloride in pure and marketed formulations. Separation was carried out using column Hypersil ODS C18 (250 mm x 4.6mm x 5μm particle size) in isocratic mode using mobile phase composition pH 6.0 ammonium acetate Buff...

Chronic pain is common in the U.S. population and one of main reasons patients seek medical care. Between 10% to 60% adults 18 years older report experiencing chronic or persistent lasting at least three six months, according a November 2021 study by Michaud colleagues published Journal Personalized Medicine. According study, more than 65% who take an opioid for also other medication concomitan...

We appreciate the recent Perspective from Nicholson and Formea (1 ) because it allows us to clarify the role of Clinical Pharmacogenetics Implementation Consortium (CPIC) guidelines for clinicians. CPIC publishes genotype-based drug therapy guidelines to help clinicians understand how genetic test results could be used to optimize drug therapy. The underlying assumption for CPIC guidelines is t...

Recently, Algeciras-Schimnich et al. highlighted the lot-to-lot variability of a quantitative immunoassay and the failure of frequently used protocols to detect these variations (1). We have discovered that lot-to-lot variations also present an important issue in qualitative immunoassays used for chronic pain monitoring. These variations impact the reliability of these assays in clinical practi...

The diverse functions of retinal amacrine cells are reliant on the physiological properties of their synapses. Here we examine the role of mitochondria as Ca(2+) buffering organelles in synaptic transmission between GABAergic amacrine cells. We used the protonophore p-trifluoromethoxy-phenylhydrazone (FCCP) to dissipate the membrane potential across the inner mitochondrial membrane that normall...

The paper by Hwang, et al. in this volume demonstrates that a reformulation of OxyContin (Purdue Pharma, Yonkers, NY, USA) in 2010, which had been intended to discourage opioid abuse, may have had the unintended consequence of discouraging its therapeutic use as well.1 This is an important finding, despite some flaws in the study, which raises important public health concerns. In this context, ...

BACKGROUND The use of opioids in the long-term management of chronic low-back pain (CLBP) has increased dramatically. Despite this trend, the benefits and risks of these medications remain unclear. This review is an update of a Cochrane review first published in 2007. OBJECTIVES To determine the efficacy of opioids in adults with CLBP. SEARCH METHODS We electronically searched the Cochrane ...

Shearers play an important role in fully mechanized coal mining face and accurately identifying their cutting pattern is very helpful for improving the automation level of shearers and ensuring the safety of coal mining. The least squares support vector machine (LSSVM) has been proven to offer strong potential in prediction and classification issues, particularly by employing an appropriate met...

The Human Genome Project heralded great hope that the clinical use of individual genetic information would result in improved care through treatments tailored to an individual patient’s genotype. Such treatment would presumably reveal individual susceptibility to chronic diseases, thus preventing them, and lead to targeted pharmacotherapies based on an individual’s metabolism and susceptibility...

Opioids have been known to have analgesic properties for thousands of years. The chronic use of opioids for the treatment of chronic noncancer pain has only lately seen increased medical practice [1] owing to a relative lack of efficacy data, until recently, from well-controlled studies. The efficacy of morphine, oxycodone and oxymorphone in extended-release dosage forms has been demonstrated i...