BACKGROUND Human telomerase reverse transcriptase (hTERT) has become an ideal target for development of anticancer therapy. Small interfering RNAs (siRNAs) are very powerful reagents for gene silencing and show promise for cancer gene therapy. However, only a small number of siRNAs have been demonstrated to be effective. For gene therapy targeting hTERT, it is essential to develop a robust syst...

1.Cline, J., J.C. Braman and H.H. Hogrefe. 1996. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerase. Nucleic Acids Res. 24:3546-3551. 2.Haas, J., E.C. Park and B. Seed. 1996. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6:315-324. 3.Higuichi, R., B. Krummel and R.K. Saiki. 1988. A general method for in vitro preparation and specific ...

criticism of public extension services has resulted in various countries seeking alternative approaches for provision of these services. in all these approaches, extension personnel are the key factors to the success of the implementation. today, agricultural extension services in turkey bear the responsibility of the ministry of food, agriculture and livestock. a number of non-public extension...

Whole-plant approaches allow quantification of the temporal overlap between primary and secondary growth. If the amount of time available to grow is short, there may be a high temporal overlap between shoot growth and wood formation. We hypothesise that such overlap depends on the duration of the growing season and relates to wood anatomy. We evaluated wood anatomy, shoot longitudinal and radia...

1.Eghtedarzadeh-Kondri, M., M.A. Walls and S.M. Glaser. 1997. Site-specific mutagenesis of immunoglobulin domains by multiple-fragment homologous recombination. BioTechniques 23:830-834. 2.Ho, S.N., H.D. Hunt, R.M. Horton, J.K. Pullen and L.R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59. 3.Huff, J.P., B.J. Grant, C.A. Penning an...