The primary purpose of the experiments reported in this paper was to gain information on the molecular origin of the mitotic apparatus. Antisera were prepared against unfertilized sea urchin (Strongylocentrotus purpuratus) egg antigens and mitotic apparatus antigens. These were permitted to react with various antigen solutions in Ouchterlony agar gel diffusion plates, and the resultant precipit...

It has been proposed that allotypic trophoblast lymphocyte cross-reactive (TLX) antigens are involved in the maintenance of normal human reproduction. Despite such a potentially important role for TLX antigens, isolation of human TLX proteins has not yet been reported. As an initial step toward elucidation of the structure and function of TLX antigens, we have isolated TLX proteins from Lubrol-...

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Antibodies directed against antigens of the asexual blood stages of Plasmodium falciparum were studied in the plasma of 29 individuals infected with P. falciparum and living in two areas of Madagascar. These plasma samples were investigated by four immunological methods: indirect fluorescence, immunoprecipitation of radiolabeled P. falciparum polypeptides, inhibition of the in vitro growth of P...

The two chicken creatine kinases from brain and from muscle have been purified as well as the chicken hybrid enzyme. The brain and muscle enzymes from the rabbit have also been purified and compared to the chicken enzymes. There are significant differences in amino acid composition between the brain and muscle types; the hybrid enzyme has an intermediate composition. Peptide maps of the muscle ...

The presence of cytochrome P-450 and associated mono-oxygenase activities was examined in brain microsomes from male and female mice. Although the cytochrome P-450 level in male mouse brain was very low as compared with mouse liver, the aminopyrine N-demethylase and morphine N-demethylase specific activities in male mouse brain were much higher than those observed in mouse liver. Ethoxycoumarin...

SUMMARY It was found that one Reuber hepatoma and seven Mor ris hepatomas with different growth rates and various degrees of differentiation contained pyruvate carboxylase (PC) activity ranging from 3 to 113% of control values. The levels of PC activity in the regenerating liver, spontane ous mouse hepatoma, and host livers of tumor-bearing rats were estimated in the range of normal rat liver. ...

Highly purified dicoumarol-induced bovine prothrombin, which does not bind calcium ions and has no prothrombin activity, has been structurally compared with normal prothrombin. Quantitative amino acid and carbohydrate analysis gave identical results for both prothrombins, as did analysis of the NHz-terminal and the COOH-terminal amino acids and molecular weight determination with the sodium dod...

Goat antibody IgG produced against bovine corpus luteum mitochondrial cytochrome P-450 (P-450scc) associated with cholesterol side chain cleavage (CSCC) was used to compare immunological characteristics of mitochondrial cytochrome P-450s from the bovine adrenal cortex (BAM), bovine corpus luteum (BCLM), and human placenta (HPM). In Ouchterlony double diffusion, anti-P450scc produced a single ba...

During last decades, diphtheria has remained as a serious disease that still outbreaks and can occur worldwide. Recently, new vaccine delivery systems have been developed by using the biodegradable and biocompatible polymers such as alginate. Alginate nanoparticles as a carrier with adjuvant and prolong release properties that enhance the immunogenicity of vaccines. In this study diphtheria tox...