Moloney murine leukemia virus reverse transcriptase (MMLV-RT) is a widely used enzyme for cDNA synthesis. Here we show that MMLV-RT has a strong template-independent polymerase activity using blunt DNA ends as substrate that generates 3' overhangs of A, C, G, or T. Nucleotides were appended efficiently in the order A > G > T > C, and tail lengths varied from 4 to 5, 2 to 7, 2 to 4, and 2 to 3 f...

The silica-based stationary phases with favorable physical characteristics are the most popular in liquid chromatography. However, there are several problems with silica-based materials: severe peak tailing in the chromatography of basic compounds, non-reproducibility for the same chemistry columns, and limited pH stability. Ionic liquids (ILs) as mobile phase components can reduce peak tailing...

The paper describes the main drive systems of an iron ore pelletizing plant and related important design aspects. It is shown how today’s drive systems provide the desired flexibility for the process and lead to high energy efficiency and system reliability. With respect to electric drives expansion projects have many technical issues in common with green-field installations. However, there are...

In this paper, the Guangxi’s red mud disposal field is taken as the study area. Appropriate calculating parameters and boundary conditions were selected based on the survey data to build a Mohr-Coul0mb and fluid model under the plane-strain condition. FLAC software was adopted to simulate the distribution of pore water pressure, filtrate seepage paths, and leakage of the dam body under conditio...

We develop a method for empirically measuring the difference in carbon footprint between traditional and online retailing (“e-tailing”) from entry point to a geographical area to consumer residence. The method only requires data on the locations of brick-and-mortar stores, online delivery points, and residences of the region’s population, and on the goods transportation networks in the studied ...

The disposal of sulfide mine tailings in an environmentally sound, yet cost-effective, manner is an issue facing most metal mines. Subaqueous tailing disposal is considered an attractive option for disposal that limits oxygen (O2) availability within sulfide mine tailings and controlling sulfide oxidation and the resultant acid mine drainage (AMD). Assuming that O2 profiles represent steady-sta...

The dc-biased optical orthogonal frequency division multiplexing (DCO-OFDM) system is experimentally demonstrated as an appealing candidate in future visible light communication (VLC) system. However, the intrinsic high PAPR drawback that the DCO-OFDM system suffers from still needs to be addressed and few effective approach has been found so far. To deal with this problem, in this paper, the t...

AIMS The objective of the study was to compare ultraviolet (u.v.) inactivation kinetics of indigenous aerobic spores in surface water with their laboratory-cultured spore isolates and to investigate the relationship between physicochemical characteristics and u.v. inactivation kinetics of spore isolates. METHODS AND RESULTS Lake water samples were analysed for the presence of indigenous aerob...

Transport of dissolved organic matter (DOM) from the Suwannee River and of synthetic polystyrene sulfonates (PSSs) was investigated in columns packed with naturally Fe/Al-oxide-coated sands from Oyster, Virginia. Surface-water samples were collected in May 2012 and processed by XAD-8 (humic substances; HPOA), XAD-4 (transphilic acids [TPIAs]), and reverse osmosis (broad range of components; NOM...

Plant small RNAs are 3' methylated by the methyltransferase HUA1 ENHANCER1 (HEN1). In plant hen1 mutants, 3' modifications of small RNAs, including oligo-uridylation (tailing), are associated with accelerated degradation of microRNAs (miRNAs). By sequencing small RNAs of the wild type and hen1 mutants from Arabidopsis thaliana, rice (Oryza sativa), and maize (Zea mays), we found 3' truncation p...