BACKGROUND Bacterial infections remain a challenge to solid organ transplantation. Due to the alarming spread of carbapenem-resistant gram negative bacteria, these organisms have been frequently recognized as cause of severe infections in solid organ transplant recipients. METHODS AND FINDINGS Between 15 May and 30 September 2012 we enrolled 887 solid organ transplant recipients in Italy with...

Tap water samples from the toilets of an Italian national railway train were collected over a period of 10 months and tested for the presence of Cyclospora cayetanensis (C. cayetanensis) using EvaGreen® real-time polymerase chain reaction (RT-PCR) assay coupled with high resolution melting (HRM) analysis for protozoan detection and oocyst quantification. C. cayetanensis positive samples were de...

UNLABELLED The walls of infectious pathogens, which are essential for transmission, pathogenesis, and diagnosis, contain sugar polymers that are defining structural features, e.g., β-1,3-glucan and chitin in fungi, chitin in Entamoeba cysts, β-1,3-GalNAc in Giardia cysts, and peptidoglycans in bacteria. The goal here was to determine in which of three walled forms of Toxoplasma gondii (oocyst, ...

A major mode of transmission of Cryptosporidium parvum, a widespread waterborne pathogen, is via contaminated drinking and recreational waters. Oocyst transport to surface water can occur by deposition of manure directly in the water or by wash off in surface runoff. Oocyst transport to groundwater is less straightforward and requires that the oocysts move through soil and bedrock to reach the ...

this experiment was designed to evaluate the effect of dietary caspian kilka fish oil on breast meat fatty acid (fa) profile and as well as sensory acceptability of breast meat. fish oil supplementation was added to provide four graded levels (0, 1, 2, and 3% fish oil) in place of vegetable oil for supporting 3% oil in the diet during the period of breeding. these four diets were allocated to o...

in the fall of 1991, the incidence of goiter was determined in i07x male and female school children in kermanshah city. the study was considered from two view points i.e., clinical examinations and measurements of levels of iodine excreted in urine, and was performed in three age groups: 7-11 years of age, 12-15 years of age and 16-18 years of age. clinical examination showed 16.5% occurrence o...

Hammondia heydorni is regarded as a protozoan parasite that uses canids, e.g. dogs and foxes, as definitive hosts, but clinical signs of infection are rare. This study therefore took advantage of the opportunity to study an oocyst population from the faeces of a dog suffering from intermittent bouts of diarrhoea. Oocysts from the naturally infected dog were shown to be H. heydorni by using the ...

background: cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. the organism can be transmitted through water. since small number of c. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of c. parvum oocyst in samples with limited number of oocysts. methods: antibody was prepared against ...