The interrelationship of five medicinally important species of Typhonium (Araceae) including T. venosum, which was previously placed under the genus Sauromatum, was inferred by analysis of random amplified polymorphic DNA (RAPD). DNA from pooled leaf samples was isolated and RAPD analysis was performed using 20 decamer oligonucleotide primers. Out of a total of 245 bands amplified, 12 were foun...

The availability of adequate information about the documentation and characterization germplasm is fundamental for any crop improvement program. importance cumin as a medicinal plant yet lack its genetic variability encouraged us to initiate current study aiming at assessing among 17 genotypes from different geographical regions using four molecular markers (RAPD, ISSR, SRAP SCoT). Further, pot...

In the present study, genetic fingerprints of ten species of Zingiberaceae from eastern India were developed using PCR-based markers. 19 RAPD (Rapid Amplified polymorphic DNA), 8 ISSR (Inter Simple Sequence Repeats) and 8 SSR (Simple Sequence Repeats) primers were used to elucidate genetic diversity important for utilization, management and conservation. These primers produced 789 loci, out of ...

Genetic variability among the genotypes of any species could be utilized for its improvement. PCR-based Random Amplified Polymorphic DNA (RAPD) technique was used to determine the genetic diversity and relationship among 10 cucumber varieties and genotypes. Five decamer primers were used to amplify genomic DNA and the primers yielded a total of 54 bands of which 36 bands were polymorphic and 18...

RAPD markers were used to reveal the genetic diversity of the genus Camptotheca. Three primers (OPA02, OPA03, and OPA04), generating 44 polymorphic bands using randomly amplified polymorphic DNA (RAPD) markers, were able to discriminate among 25 Camptotheca populations. The band size varied from 268-4,411bp, with an average of 15 bands/primer. Of these populations, cultivars ‘Katie’ (KT) and ‘A...

A study was undertaken to evaluate the genetic variation in the 10th generation of a breeder flock of native breed selected for high egg and meat production in native fowls breeding station, Mazandaran, Iran. Venous blood samples were collected from 100 birds of both sexes. The RAPD-PCR technique was applied to generate a DNA fingerprint of individuals. Initially, a total of 20 ten-nucleotide a...

Alfalfa is an autotetraploid, allogamous and heterozygous forage legume, whose varieties are synthetic populations. Due to the complex nature of the species, information about genetic diversity of germplasm used in any alfalfa breeding program is most beneficial. The genetic diversity of five alfalfa varieties, involved in progeny tests at Institute of Field and Vegetable Crops, was characteriz...

The breeding material comprising 17 Iraqi wheat cultivars belongs to three different types, i.e., a) salinity-tolerant (Dajla, Furat, 1H, 2H, 2N, 3H, 3N, and 7H), b) drought-tolerant (Sham-6 Orok), c) local (Iraq, Iba99, Iba95, Abu Ghraib-3, Adnanin, Tamoze, Alrashid) underwent qualitative characteristics genomic analysis studies in 2021-2022, at the Biotechnology Research Center, Al-Nahrain Un...

We described the use of the random amplified polymorphic DNA (RAPD) technique on Plasmodium falciparum DNA to detect genetic markers for chloroquine-resistant strains. Fourteen RAPD primers were tested, three of which generated banding patterns correlated with chloroquine resistance. To measure this correlation, the RAPD profiles were analyzed using the Nei and Li similarity coefficient. Detect...

Random amplification of polymorphic DNA (RAPD) analysis using the polymerase chain reaction proved a useful technique in the biodiversity analysis of microorganisms but may lack reproducibility in the poorly standardized methodology. In the present investigation, the RAPD technique was optimized for characterizing cyanobacterial isolates in order to ensure its reproducibility and discriminatory...