A quantitative real-time polymerase chain reaction assay to detect and quantify a portion of the outer membrane protein B gene (ompB) of Rickettsia amblyommii was employed to assess the threat of R. amblyommii exposure to humans parasitized by Amblyomma americanum (the lone star tick). A total of 72 pools of lone star ticks removed from humans were acquired from two collections and used in this...

Nature has provided a complete storehouse of remedies to cure ailments of mankind. About 80% of the world's population depends wholly or partially on traditional medicine for its primary health care needs. Pharmacognosy is mainly concerned with naturally occurring substances having a medicinal action. Morphological and anatomical characters play a vital role in crude drug standardization. Howev...

The antibacterial activity of essential oils extracted from medicinal plants (Ocimum gratissimum, L., Cybopogum citratus (DC) Stapf., and Salvia officinalis, L.) was assessed on bacterial strains derived from 100 urine samples. Samples were taken from subjects diagnosed with urinary tract infection living in the community. Microorganisms were plated on Müller Hinton agar. Plant extracts were ap...

Plants have been used for the treatment of various diseases for thousands of years. Plants and plant-products are good sources of medications and provide raw materials for modern pharmaceuticals used for various ailments. Ocimum sanctum and Ocimum gratissimumis known to be an important medicinal plants from the ancient period in India. Thisstudy was carried out to investigate the phytochemical ...

Objectives The objectives of the present study were to determine the duration of infestation by Amblyomma americanum necessary for transmission of Cytauxzoon felis to domestic cats and to determine if ingestion of C felis-infected A americanum by cats is a route of transmission. Methods Forty-nine cats were assigned to one of seven groups, with seven cats per group. Cats were infested with A am...

The γ-proteobacterium Francisella tularensis causes seasonal tick-transmitted tularemia outbreaks in natural rabbit hosts and incidental infections in humans in the south-central United States. Although Dermacentor variabilis is considered a primary vector for F. tularensis, Amblyomma americanum is the most abundant tick species in this endemic region. A systematic study of F. tularensis coloni...

Eugenol (C10H12O2) presents various therapeutic properties. Due to this fact, it arises interest into the pharmaceutic industry and has a commercial value. However, there is still low offer of product in market. The aim work was evaluate concentration essential oil from dried leaves O. gratissimum L., determine whether species bares promising characteristics as source eugenol. Fresh Ocimum L. w...

Only indirect or circumstantial evidence has been published to support transmission of Rickettsia rickettsii by Amblyomma americanum (lone star) ticks in North America. This study provides molecular evidence that A. americanum ticks can function, although most likely infrequently, as vectors of Rocky Mountain spotted fever for humans.