OBJECTIVES In the ob/ob mouse model of obesity, chronic absence of leptin causes a significant increase of orexin (OX) production by hypothalamic neurons and excessive food intake. The altered OX level is linked to a dramatic increase of the inhibitory innervation of OX producing neurons (OX neurons) and the over expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) by OX neurons of o...

[This corrects the article DOI: 10.1371/journal.pone.0160431.].

Diabetes alters cardiac substrate metabolism. The cardiac phenotype in insulin-resistant states has not been comprehensively characterized. The goal of these studies was to determine whether the hearts of leptin-deficient 8-week-old ob/ob mice were able to modulate cardiac substrate utilization in response to insulin or to changes in fatty acid delivery. Ob/ob mice were insulin resistant and gl...

Recent epidemiological, clinical, and experimental studies have demonstrated important links between sleep duration and architecture, circadian rhythms, and metabolism, although the genetic pathways that interconnect these processes are not well understood. Leptin is a circulating hormone and major adiposity signal involved in long-term energy homeostasis. In this study, we tested the hypothesi...

The mutant gene responsible for obesity in the ob/ob mouse was recently identified by positional cloning (Zhang Y., R. Proenca, M. Maffel, M. Barone, L. Leopold, and J.M. Friedman. 1994. Nature (Lond.) 372:425). The encoded protein and to represent and "adipostat" signal reflecting the state of energy stores. We confirm that the adipocyte is the source of ob mRNA and that the predicted 16-kD ob...

Laposky, Aaron D., Jonathan Shelton, Joseph Bass, Christine Dugovic, Nicholas Perrino, and Fred W. Turek. Altered sleep regulation in leptin-deficient mice. Am J Physiol Regul Integr Comp Physiol 290: R894–R903, 2006. First published November 17, 2005; doi:10.1152/ajpregu.00304.2005.—Recent epidemiological, clinical, and experimental studies have demonstrated important links between sleep durat...

The neuroendocrine effects of leptin on metabolism hold promise to be translated into a complementary therapy to traditional insulin therapy for diabetes and obesity. However, injections of leptin can provoke inflammation. We tested the effects of leptin, produced in the physiological adipocyte location, on metabolism in mouse models of genetic and dietary obesity. We generated 3T3-L1 adipocyte...

We numerically analyze Non-Oberbeck-Boussinesq (NOB) effects in two-dimensional Rayleigh-Bénard flow in glycerol, which shows a dramatic change in the viscosity with temperature. The results are presented both as functions of the Rayleigh number Ra up to 10 (for fixed temperature difference ∆ between the top and bottom plates) and as functions of ∆ (“nonOberbeck-Boussinesqness” or “NOBness”) up...

KAPLAN, MURRAY L., AND GILBERT A. LEVEILLE. Core temperature, 02 consumption, and early detection of ob/ob genotype in mice. Am. J. Physiol. 227(4) : 912-915. 1974.-Oxygen consumption and core temperature of ob/ob mice and their thin littermates were measured at various ambient temperatures from 10 to 30°C. At every ambient temperature studied, adult obese mice exhibited lower 02 consumptions a...