Microsomes from rat liver were extracted by low ionic strength solutions. Extracted microsomes lost most of the linoleic acid desaturation activity. The addition of the extract back into the extracted microsomes was necessary to restore full desaturation activity. The soluble fraction had no desaturation activity. The existence of a soluble factor loosely bound to the microsomes, stable to soni...

In this study, the malondialdehyde (MDA) levels in the muscle, liver and kidney tissues and the antioxidant vitamin (A, C, E) levels in the muscle tissue of juvenile rainbow trout were investigated under different oxygen levels (3.5 mg O2/l, 4.5 mg O2/l and 7 mg O2/l) at the end of the 8 hour trial. Fish (initial weight and length, 30.19±2.2 g and 13.15±0.22 cm, respectively) were distributed i...

In this study, the malondialdehyde (MDA) levels in the muscle, liver and kidney tissues and the antioxidant vitamin (A, C, E) levels in the muscle tissue of juvenile rainbow trout were investigated under different oxygen levels (3.5 mg O2/l, 4.5 mg O2/l and 7 mg O2/l) at the end of the 8 hour trial. Fish (initial weight and length, 30.19±2.2 g and 13.15±0.22 cm, respectively) were dis...

A total of 155 consecutive anaesthetics in three public Malaysian hospitals were prospectively studied to assess preoxygenation practices by their anaesthesia providers. Preoxygenation was practised in 96.1% of patients. Specialist and non-specialist anaesthesiologist did not preoxygenate 8.8% and 2.3% of their patients, respectively. Overall incidence of arterial oxygen desaturation during ind...

Objective: In this study, we aimed to investigate the incidence of cerebral desaturation and possible relationship between intraoperative postoperative complications. Methods: A prospective, observational study was performed 115 patients under 18 years age who required open heart surgery in a university hospital. Cerebral defined as 25% decrease saturation (low alarm limit) when compared with b...

Intact spinach (Spinacia oleracea) chloroplasts, pulse-labeled with [(14)C]acetate, desaturate newly formed fatty acids as ester groups of monogalactosyl diacylglycerol in a subsequent chase in the dark. Rupture of pulse-labeled chloroplasts by addition of a detergent solution 3-([3-cholamidopropyl]dimethylammonio)-1-propane sulfonate preserves part of this desaturation activity. Direct additio...

Stearoyl-CoA desaturase (SCD) is expressed at high levels in several human tissues and is required for the biosynthesis of oleate (18:1) and palmitoleate (16:1). These monounsaturated fatty acids are the major components of phospholipids, triglycerides, wax esters, and cholesterol esters. Mice with a targeted disruption of the SCD1 gene have very low levels of VLDL and impaired triglyceride and...