PCR-based detection techniques enables reliable and sensitive nucleic acid target detection. However, quantitative determination methods often fail to control for the efficiency of nucleic acid extraction, reverse transcription, and PCR amplification. This problem is even more prominent when working with clinical samples due to target sequence loss during nucleic acid processing or the co-purif...

In this paper, we developed a metal micro-fluidic chip with advanced surface processing for ultra-small volume molecular isothermal amplification. This method takes advantages of the nucleic acid amplification with good stability and consistency, high sensitivity about 31 genomic DNA copies and bacteria specific gene identification. Based on the advanced surface processing, the bioreaction assa...

The SoGAT working group for the safety testing of blood, tissues and organs for blood-borne pathogens has played an important role in the standardisation of nucleic acid amplification techniques (NAT) for blood safety. Regular meetings of this group have taken place since 1995, and have provided a valuable and unique forum for the development of WHO International Standards and other reference r...

Received: Revised: Accepted: 2014–05–26 2014–07–14 2014–07–15 Disease diagnosis is of crucial importance in making appropriate therapeutic decisions. Conventionally, diagnosis was based on isolation and identification of the causative agent. This was followed in timeline by the development of serological tests. Nowadays, molecular tests credited with high sensitivity like polymerase chain react...

Abstract With an almost unremittent progression of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections all around the world, there is a compelling need to introduce rapid, reliable, and high-throughput testing allow appropriate clinical management and/or timely isolation infected individuals. Although nucleic acid amplification (NAAT) remains gold standard for detecting theo...

BACKGROUND The diagnosis of active pulmonary tuberculosis (TB) remains a challenge in clinic, especially for sputum negative pulmonary TB. Bronchoalveolar lavage fluid (BALF) has higher sensitivity than sputum for detection of Mycobacterium tuberculosis (Mtb). However, bronchoscopy is invasive and costly, and not suitable for all patients. In order to make TB patients get more benefit from BALF...

Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease. The next generation of diagnostic devices will interrogate the genetic determinants of such conditions at the point-of-care, affording clinicians prompt reliable diagnosis from which to guide more effective treatment. The complex biochemical nature of clinical samples, the l...

The Australian Mycobacterium Reference Laboratory Network collected and analysed laboratory data on new cases of disease caused by Mycobacterium tuberculosis complex in the year 2002. A total of 712 cases were identifi ed by bacteriology, representing an annual reporting rate of 3.6 cases of laboratory-confi rmed tuberculosis per 100,000 population. The most commonly encountered culturepositive...