The mechanisms of liver damage in chronic hepatitis C virus (HCV) infection are poorly understood. The transcription factor, nuclear factor-kappa B (NF-kappa B), regulates the expression of genes involved in apoptosis, inflammation, and antiviral response. It plays a protective role in several forms of liver damage. In this study, we analyzed NF-kappa B by gel mobility shift assay and immunohis...

Interleukin (IL)-10, a multifunctional immune-regulatory cytokine with both immunosuppressive and anti-angiogenic functions, is produced by immune cells including macrophages, T lymphocytes, and natural killer cells. Among other effects, IL-10 promotes tumor cell proliferation and metastasis via immunosuppression. Interleukin-10-mediated immunosuppression is aided by synthesis of tumor necrosis...

Background and Aims: Diabetes mellitus causes testicular damage by increasing oxidative stress and inflammation. In the present study, modulation of oxidative stress by pioglitazone, a synthetic ligand of peroxisome proliferator-activated receptor-γ, was examined in testis of streptozotocin-induced diabetic rats. Materials and Methods: Diabetes was induced by a single dose of streptozot...

Oxidative stress has been proposed as a common mediator of apoptotic death. To investigate further the role of oxidants in this process we have studied the effects of antioxidants on Sindbis virus (SV)-induced apoptosis in two cell lines, AT-3 (a prostate carcinoma line) and N18 (a neuroblastoma line). The thiol antioxidant, N-acetylcysteine (NAC), at concentrations above 30 mM, completely abro...

This paper reports a simple, rapid molecular binding scheme for the detection and quantification of the transcription factor nuclear factor kappa B and other DNA binding proteins without any separation or immobilization step.

objective(s): to study the effect of cardiopulmonary bypass (cpb) on nuclear factor-kappa b (nf-кb) and cytokine expression and pulmonary function in dogs. materials and methods: twelve male mongrel dogs were divided into a methylprednisolone group (group m) and a control group (group c). all animals underwent aortic and right atrial catheterization under general anesthesia. changes in pulmonar...

Cells of the monocyte-macrophage lineage are important targets of HIV infection. We report here that the phenotypic differentiation of monocyte cell lines induced by phorbol esters or tumour necrosis factor alpha (TNF alpha) is associated with expression of nuclear factor kappa B (NF-kappa B). In parallel with such differentiation, HIV transcription, monitored using an HIV long terminal repeat ...