Ras signaling is tightly regulated during neural stem cell (NSC) differentiation, and defects in this pathway result in aberrant brain development. However, the mechanism regulating Ras signaling during NSC differentiation was unknown. Here, we show that stabilized HRas specifically induces neuronal differentiation of NSCs. Lentivirus-mediated HRas overexpression and knockdown resulted in stimu...

Accumulation of α-synuclein (α-syn) into insoluble aggregates occurs in several related disorders collectively referred to as synucleinopathies. To date, studies have used neural stem cells (NSCs) to examine questions about α-syn propagation, but have overlooked the therapeutic potential of NSC transplantation to modulate cognition in disorders such as dementia with Lewy bodies or Parkinson's d...

We have designed a novel transcriptome subtraction method for the genome-scale analysis of differential gene expression in highly complex eukaryotes, in which suppression subtractive hybridization (SSH) is performed first to enrich the target and, after exchange of adapters, negative subtraction chain (NSC) is then used to eliminate the remaining background. NSC evolved from differential subtra...

As part of the Measuring Black Holes in Below Milky Way-mass (M$^\star$) galaxies (MBHBM$^\star$) Project, we present a dynamical measurement supermassive black hole (SMBH) mass nearby lenticular galaxy NGC 3593, using cold molecular gas $^{12}$CO(2-1) emission observed at an angular resolution $\approx0''.3$ ($\approx10$ pc) with Atacama Large Millimeter/submillimeter Array (ALMA). Our ALMA ob...

The excessive electrical activity of neurons is reported in many diseases including: Parkinson's disease, Alzheimer's disease, and Epilepsy. Electrical overactivity in hippocampus accelerates the depletion of neural stem cell (NSC) and impairs the neurogenesis in hippocampus. It is believed that neurogenesis in hippocampus improves the cognitive functions. In this experiment, we use kindled mod...

Abstract This article presents a numerical study on the influence of anchorage shear hysteresis seismic response nonstructural components (NSC) connected to multi-storey reinforced concrete (RC) buildings, and itself. To cover variety different types shapes, this contribution considered experimental results obtained for five post-installed anchors. The were used calibrating model connecting an ...

A new antitumor antibiotic, fredericamycin A (FCRC-A48, NSC-305263), has been isolated from a strain of Streptomyces griseus (FCRC-48). Based on its unique ultraviolet-visible spectrum, infrared spectrum, proton and carbon-13 nuclear magnetic resonance spectra and mass spectra, it is judged to be a novel acid-base indicator type of compound. Its production, isolation and physicochemical propert...

Spinal cord injury (SCI) is a common and potentially life-threatening condition with no established treatment to treat the primary injury. Mesenteric neural stem cell (NSC) therapy promising SCI in chronic phase. We aimed review literature narratively describe current evidence regarding mesenteric NSC SCI. Primary refers tissue damage that occurs at time of trauma leads death neuronal cells. In...

BACKGROUND Rett Syndrome (RTT) is an Autism Spectrum Disorder and the leading cause of mental retardation in females. RTT is caused by mutations in the Methyl CpG-Binding Protein-2 (MECP2) gene and has no treatment. Our objective is to develop viral vectors for MECP2 gene transfer into Neural Stem Cells (NSC) and neurons suitable for gene therapy of Rett Syndrome. METHODOLOGY/PRINCIPAL FINDIN...

Grain-filling is a crucial process that determines final grain yield in rice (Oryza sativa L.). To understand the genetic basis of dynamics of grain-filling, quantitative trait locus (QTL) analysis was conducted using time-related phenotypic data on grain-filling collected from a population of 155 recombinant inbred lines (F12), derived from a cross between Milyang 23 and Akihikari. Two QTLs de...