The hybridization signature approach, using colony filters and labeled complex probes, can provide high throughput measurement of gene activity. We describe here the implementation of this method to follow the expression levels of 47 genes in resting and activated T cells, as well as in epithelial cells. Using 4-fold spotting of colonies, imaging plate detection and various correction and norma...

Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utilized EDTA, LSS, and CTAB to successfully inhibit RNase activities. It was found ...

OBJECTIVE To determine the burden of hospitalised, radiologically confirmed pneumonia (World Health Organization protocol) in Northern Territory Indigenous children. DESIGN, SETTING AND PARTICIPANTS Historical, observational study of all hospital admissions for any diagnosis of NT resident Indigenous children, aged between > or = 29 days and < 5 years, 1 April 1997 to 31 March 2005. INTERVE...

Evidence shows that Aboriginal and Torres Strait Islander people have the highest national percentage of morbidity in relation to diabetes. Aboriginal and Torres Strait Islander people also suffer the greatest risk of amputation as a complication of diabetes. This participatory action research project sought to discover the opinions of a range of people, including registered nurses, general pra...

A novel transcription system was constructed that allows trimming of 3' termini of RNA transcripts in E. coli by endogenous RNase P. Here, the sequence of tRNASer from E. coli fused downstream of the target sequence directs posttranscriptional cleavage 3' of the target sequence. As a first-target MNV11(+), a self-replicating RNA from the QB system was subjected to transcription in vivo. Norther...

DATA AND METHODS The data for Northern Ireland for the years 1922-1978 were obtained from the Annual Reports of the Registrar General for Northern Ireland'. For the years 1913 to 1921 the data were obtained from the Second Annual Report of the Registrar General (Northern Ireland), 19232. Provisional data for 19793 were provided by the General Register Office for Northern Ireland. The data for t...

INTRODUCTION Although research interest in women's health is growing, much of the literature does not sufficiently describe the importance of geography and gender for the health of women. This qualitative study explored factors in the northern Canadian context that influence women's health by interviewing 25 women in northern Canada. RESULTS Findings reveal that the importance of the northern...

Deep sequencing studies frequently identify small RNA fragments of abundant RNAs. These fragments are thought to represent degradation products of their precursors. Using sequencing, computational analysis, and sensitive northern blot assays, we show that constitutively expressed non-coding RNAs such as tRNAs, snoRNAs, rRNAs and snRNAs preferentially produce small 5' and 3' end fragments. Simil...

Aboriginal mobility and the sustainability of communities: Case studies from northwest Queensland and eastern Northern Territory Aboriginal mobility and the sustainability of communities: Desert Knowledge CRC Working Paper #5 Information contained in this publication may be copied or reproduced for study, research, information or educational purposes, subject to inclusion of an acknowledgement ...

northern blots or more extensive analyses using microarrays, can also be used. For these latter methods, the isolation and quantitation of intact total RNA are prerequisites for successfully measuring the level of reduction of the mRNA transcript associated with the siRNA treatment. In this article, we discuss how the Experion automated electrophoresis system, which uses microfluidics technolog...