The dissociation constants of the following substituted phenols in 50 wt percent methanol have been measured at 25 °C: o-nitrophenol, p-nitrophenol, 4-methyl-2-nitrophenol, 2,6-di chlorophenol , pentachlorophenol. The respective values found, expressed as pK values, are 7.91" 7.692 , 8.28" 7.680 , and 5.27.. The dissociation cons tants of 4-methyl-2-nitrophenol and pentachlorophenol, again exp ...

Nitrophenol is one of the most highly toxic heavy pollutants in environment. It has a slow degradation rate, and harmful to humans even at low concentrations. Therefore, it crucial detect nitrophenol quickly, easily, accurately. In this study, isomers (o-nitrophenol, m-nitrophenol, p-nitrophenol), solid liquid phases with methanol or acetone as solvent, were investigated using Raman spectroscop...

We generated purine nucleoside phosphorylase (PNP)-deficient mice to gain insight into the mechanism of immune deficiency disease associated with PNP deficiency in humans. Similar to the human disease, PNP deficiency in mice causes an immunodeficiency that affects T lymphocytes more severely than B lymphocytes. PNP knockout mice exhibit impaired thymocyte differentiation, reduced mitogenic and ...

We have discovered that the gerbil's chorda tympani nerve response to sucrose is suppressed by p-nitrophenyl alpha-D-glucopyranoside (PNP-Glu) and chloramphenicol (CAP). Mixture experiments of PNP-Glu and CAP with sodium chloride, potassium chloride, hydrochloric acid, and sucrose gave rise to the following observations: Neither PNP-Glu nor CAP alone stimulates the gerbil's taste nerve; while t...

The biodegaradation of p-nitrophenol was studied experimentally at different initial p-nitrophenol concentrations under different environmental and operational conditions (like incubation temperature, pH, inoculum size etc.) by using microbial strain Arthrobacter chlorophenolicus A6. The rate of degradation of pnitrophenol was observed maximum at incubation temperature 30 ̊C and pH 7 ± 0.2, inoc...

Pada saat ini masyarakat tengah menghadapi musim Pandemic Covid-19 khususnya di Palangka Raya, Kalimantan Tengah yang menyebabkan perilaku bertransportasi pun berubah, pengguna dan penyelenggara/operator transportasi perlu beradaptasi dengan kebiasaan baru (new normal) dilakukan pembatasan jumlah penumpang sebesar 50%. Penelitian bertujuan untuk mengetahui besarnya Biaya Operasional Kendaraan, ...

Purine nucleoside phosphorylase (PNP) catalyzes the synthesis and phosphorolysis of purine nucleosides, interconverting nucleosides with their corresponding purine base and ribose-1-phosphate. While PNP plays significant roles in human and pathogen physiology, we are interested in developing PNP as a catalyst for the formation of nucleoside analog drugs of clinical relevance. Towards this aim, ...

Purine nucleoside phosphorylase (PNP; EC: 2.4.2.1) is a key enzyme involved in the purine salvage pathway. A recent bioinformatic study by Yadav, P. K. et al. (Bioinformation 2012, 8(14), 664-672) reports PNP as an essential enzyme and potential drug target in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). We conducted an analysis using the methodology outlined by the...

Purine nucleoside phosphorylase (PNP) in mammalian tissue is an enzyme responsible for formation of purine bases in DNA. It is also believed that PNP is crucial under energy-deprived conditions for the cell to metabolise adenosine during ATP degradation. This work describes a new method for determination of PNP activity in myocardial tissue using a commercially available substrate, 2-amino-6-me...

The compounds in diesel exhaust particles (DEP) that are responsible for vasodilatation were isolated and characterized for the first time. From benzene extract of DEP, 2-methyl-4-nitrophenol, 3-methyl-4-nitrophenl and 4-nitrophenol were isolated, and their vasodilatation activities were confirmed. 3-methyl-4-nitrophenol caused dilatation of rat thoracic artery, and the other two nitrophenols, ...