MOTOMIYA, M., SATO, H., ARAI, H., NAGAI, H., ITO, M., SATOH, K., KUMANO, N. and Koxxo, K. Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG. Tohoku J. exp. Med., 1981, 133 (3), 293-300 A hot-water extract from delipidated Mycobacterium bovis strain BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfrac...

The lichen Anaptychia fusca and several Stereocaulon species have been chemically investigat­ ed with regard to their content o f unknown ninhydrin-positive compounds. N ew aliphatic and alicyclic, amino components isolated from water extracts o f these lichen species have been charac­ terised as aminopentitol, aminohexitol and aminocyclitetrol. To our knowledge, this is the first time that com...

Three selective, inexpensive and validated methods were developed and optimized for determination of antiepileptic drug, topiramate in drug substance and drug product as well as in the presence of its acid, base and thermal degradants. The first method is micelle-enhanced spectrofluorimetric assay based on the reaction between the studied drug and fluorescamine to give highly fluorescent deriva...

The presence of amino acid nitrogen in the non-protein nitrogen fraction of blood plasma was first established in 1912 by Van Slyke and Meyer (1). Chemical, microbiological, and chromatographic techniques have subsequently been employed to determine many of the individual amino acids which contribute to the total or-amino nitrogen as currently measured by the ninhydrin-CO2 method of Hamilton an...

The host-specific toxin of Helminthosporium carbonum (C(32)H(50)N(6)O(10)) was hydrolyzed by 6 n HCl to yield a number of alpha-amino acids. The common amino acids, proline and alanine, occurred in a ratio of 1:2. Two other unstable alpha-amino acids that produced lower color values with ninhydrin were also produced. One of these was tentatively identified as 2-amino-2,3-dehydro-3-methylpentano...

Phospholipid composition of 10 Bifidobacterium strains of human intestinal origin and of 9 Lactobacillus strains was determined by quantitative two-dimensional thin-layer chromatography. Phospholipids of three Bifidobacterium strains from honey bees and of two strains from bovine rumen liquor were qualitatively investigated. Diphosphatidylglycerol and phosphatidylglycerol were present in strain...