er than the normal amount. Especially, it is interesting that nicotinic acid reduces serum cholesterol and triglycerides.i) To study the unknown mechanism, the establishment of a practical method to measure nicotinic acid and its direct metabolite, nicotinuric acid, is important. In l978, Hengen et al.2] measured nicotinic acid and nicotinuric acid in plasma and urine with reversed-phase high-p...

During the course of horseradish peroxidase-mediated oxidation of either o-dianisidine or 2-2'-azino-di(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS), no O2 consumption took place. When isonicotinic acid hydrazide (isoniazid) (INH) was included in the reaction mixture, O2 was consumed in amounts linearly related to the INH concentration. Nicotinic acid hydrazide at equimolar concentrations ind...

Bacteria able to grow with nicotinic acid as a sole source of carbon and nitrogen have been isolated by a number of investigators (l-5). Hughes et al. (6-10) found that Pseudomonas jlzwrescens initiates degradation of nicotinic acid by a hydroxylation to form 6-hydroxynicotinic acid and established that the responsible enzyme is located in a cell wall membrane fraction. An initial hydroxylation...

The first two enzymes employed by a Bacillus species for the dissimilation of nicotinic acid are coordinately induced. The inducer of the enzymes appears to be 6-hydroxynicotinic acid, the product of the first enzyme in the pathways. Synthesis of the enzymes is repressed by glucose when ammonium is present in the medium, but not when nicotinic acid is the sole nitrogen source. The possible sign...

One of the first metabolic products of tryptophan to be reported was kynurenic acid, which was isolated by Ellinger in 1904 (1) from the urine of dogs that had been fed the amino acid. He characterized the compound correctly except for the assignment of the carboxyl group to the 3 position in the quinoline ring, but its correct location at the 2 position was determined soon afterward (2). Anoth...

Nicotinamide-adenine dinucleotide (NAD+) synthetases catalyze the last step in NAD+ metabolism in the de novo, import, and salvage pathways that originate from tryptophan (or aspartic acid), nicotinic acid, and nicotinamide, respectively, and converge on nicotinic acid mononucleotide. NAD+ synthetase converts nicotinic acid adenine dinucleotide to NAD+ via an adenylylated intermediate. All of t...

Lactobacillus arabinosus 17-5 has been widely used as an assay organism for nicotinic acid (NA) since the development of the method by Snell and Wright (1). Although it has been realized that other substances present in tissue extracts may interfere with the bioassay, the nature of such substances has not been elucidated. During an investigation of tryptophan metabolism in the pea plant, we stu...

During the germination of seeds and the early growth of plants, trigonelline (N-methylnicotinic acid) disappears and pyridine nucleotides appear in the plant tissue (1, 2). Hence, it has been surmised, albeit without direct evidence, that the trigonelline of the seeds is a precursor of pyridine nucleotides in seedling plants. In an earlier study from this laboratory (3), it was shown that prepa...