Soluble extracts of Neurospora crassa contain a single, electrophoretically distinct, superoxide dismutase. This enzyme has been isolated and has been found to be a bluegreen, copperand zinc-containing enzyme, similar to that already described from bovine tissues and from garden peas. The molecular weight was ,approximately 31,000, and the enzyme appeared to be composed of 2 subunits of equal s...

Fungi respond and adapt to many environmental signals including light. The photobiology of fungi has been extensively investigated, but in recent years the identification of the first fungal photoreceptor, WC-1 in the ascomycete Neurospora crassa, and the discovery that similar photoreceptors are required for photoreception in other ascomycete, basidiomycete and zygomycete fungi has allowed the...

We have developed soluble extracts from Neurospora crassa capable of accurately initiating the transcription of cloned Neurospora protein-encoding genes by RNA polymerase II in vitro. The genes encoding glutamate dehydrogenase (am) and histones H3 and H4 were transcribed by the extracts, and transcription was sensitive to alpha-amanitin at 1 mg/ml. The 5' heterogeneity of the in vitro initiatio...

13. KOCH, L. H. and McMEEKIN, T. L. A new direct nesslerization microkjeldahl method and a modification of the nessler-folin reagent for ammonia. Jour. Amer. Chem. Soc. 46: 2066-2068. 1924. 14. KVAMME, E. and HELLMAN, L. Isolation of pyruvic and alpha-ketoglutaric acids from blood and tissues in the presence of carbon-14 acetate. Anal. Chem. 26: 1995-1997. 1954. 15. OWENS, R. G. Metabolism of f...

The isolation and characterization of the circular mitochondrial plasmid pUG1 from the ascomycete Cryphonectria parasitica is described. The entire sequence (4182 bp) was obtained and high similarities to DNA-dependent DNA polymerases were revealed. Strikingly common features with the DNA polymerases encoded by the Neurospora intermedia plasmids Fiji and LaBelle, such as matches to the conserve...

Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Effect of polyene antibiotics on protoplasts of Neurospora crassa. J. Bacteriol. 83:351-358. 1962.-The polyene antibiotics nystatin, amphotericin B, and filipin induced shrinkage of Neurospora crassa protoplasts stabilized in sucrose. At low concentrations of filipin (5 mug per ml), or higher concentrations of nystatin and amphotericin...

Although the role of carbon dioxide in metabolism has been examined in an extensive variety of animal and plant tissues (l), especially in microorganisms (2), to the authors’ knowledge the literature is devoid of any such references for Neurospora. This mold is particularly appropriate for such studies in view of the many well known mutants of diverse metabolic potentialities which might reveal...

A Spore killer trait, which exhibits genetic and cytological properties analogous to those previously found in Neurospora, exists in natural populations of the fungal plant pathogen Fusarium moniliforme. The genogeography of the polymorphism in F. moniliforme differs from the situation in Neurospora intermedia. It is more akin to the situation in N. sitophila, although more extreme with respect...

In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination chemo-catalysis and multi-enzymatic in vivo cascade reaction development highly versatile high-throughput assay for enzymatic reduction carboxylic acids. We investigated biocompatible metal-catalyzed preparation ? or ? substituted cinnamic acid derivatives were fed directly into biocatalyti...