INTRODUCTION Yellow fever is considered a re-emerging disease and is endemic in tropical regions of Africa and South America. At present, there are no standardized or commercialized kits available for yellow fever virus detection. Therefore, diagnosis must be made by time-consuming routine techniques, and sometimes, the virus or its proteins are not detected. Furthermore, co-circulation with ot...

A nested polymerase chain reaction (PCR) assay was used to detect early stages of Myxobolus cerebralis in caudal and adipose fin samples from rainbow trout (RT). To determine sensitivity, groups of 10 RT were exposed to 2,000 M. cerebralis triactinomyxons/fish for 1 hour at 15 degrees C and subsequently moved to clean recirculating water. Fish were held for 2 and 6 hours and 1, 2, 3, 5, 7, 10, ...

Genotyping 42 serum samples taken from the pigs in the oubreaks from 2009 to 2013 with RT-PCR and nested RT-PCR, showed more than 80% of samples were positive with Chinese PRRSV clade, and the others were European and North American classical PRRSV genotypes. Ten serum samples from unnapprent PRRS herds were examined for antibodies against PRRSV with ELISA and also for PRRSV with RT-PCR. It was...

The prevalence, virological and epidemiological aspects of the hepatitis C virus (HCV) and the hepatitis B virus (HBV) infections vary among hemodialysis patients in different countries. Aiming at analyzing these aspects of HCV and HBV infections in hemodialysis patients in Belo Horizonte, MG, Brazil, we studied three hemodialysis units including 434 patients. Serology was used to detect anti-H...

objective: chronic myeloid leukemia (cml) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. investigations have shown different types of bcr- abl variants in these patients. in iran only 3 types of these variants have been identified because most of the clin...

Dengue virus and dengue haemorrhagic fever are amongst the most important challenges in tropical diseases due to their expanding geographical distribution, increasing outbreak frequency, hyperendemicity and evolution of virulence. Here, the use of a RT-nested PCR for both the diagnosis and genetic characterization of dengue infections in clinical samples is described.

Prevalence of asymptomatic norovirus infection was investigated in food handlers in South Korea. Among 6,441 subjects, 66 (1.02%) had norovirus infections confirmed by reverse transcription (RT)-PCR (real time and nested). GII-12 and GII-4 were the prevalent genotypes. Our data suggest that infection of asymptomatic food handlers is an important transmission source in norovirus outbreaks.