نتایج جستجو برای: nested polymerase chain reaction nested

تعداد نتایج: 707924  

2017
Partha Sarathi Mandal Sunit Kumar Mukhopadhayay Saktipada Pradhan Samiran Mondal Chandrakanta Jana Nimai Chandra Patra Rabindra Nath Hansda

AIM The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference to standardize nested polymerase chain reaction (PCR). MATERIALS AND METHODS After postmortem of suspected carcasses, samples were collected fo...

2010
Frank J M van Kuppeveld Arjan S de Jong Kjerstin H Lanke Gerald W Verhaegh Willem J G Melchers Caroline M A Swanink Gijs Bleijenberg Mihai G Netea Jochem M D Galama Jos W M van der Meer

OBJECTIVE The presence of the retrovirus xenotropic murine leukaemia virus-related virus (XMRV) has been reported in peripheral blood mononuclear cells of patients with chronic fatigue syndrome. Considering the potentially great medical and social relevance of such a discovery, we investigated whether this finding could be confirmed in an independent European cohort of patients with chronic fat...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2013
Diógenes Rodrigues Fernanda de-Paris Rodrigo Minuto Paiva

INTRODUCTION Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. METHODS Serial dilutions of quantified commercial controls of each ...

Journal: :Memorias do Instituto Oswaldo Cruz 2006
Cecília Eugenia Charbel José Eduardo Levi José Eduardo Costa Martins

The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM ...

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