Respiratory tract infections can be caused by a heterogeneous group of viruses and bacteria that produce similar clinical presentations. Specific diagnosis therefore relies on laboratory investigation. This study developed and evaluated five groups of multiplex nested PCR assays that could simultaneously detect 21 different respiratory pathogens: influenza A virus (H1N1, H3N2, and H5N1); influe...

Background: This study aimed to design and analyze the applicability of an oligonucleotide probe in radioisotope 32P-based dot blot hybridization for detection of hepatitis C virus. Methods: Forty-six of plasma samples were used. The plasma was extracted to obtain viral RNA genome as template for RT-PCR and the amplicon was used for nested PCR. Twenty-four HCV genomes were retrieved from GeneBa...

objective: gbv-c is a virus transmissible via blood transfusion and sexual routes. because of the shared transmission routes in gbv-c and hepatitis b virus (hbv) we have attempted to detect gbv-c in hbsag-positive patients using the more sensitive semi-nested pcr. we used restriction fragment length polymorphism (rflp) to determine genotype. methods: a total of 100 serum samples were collected...

babesiosis is an important tickborne disease in the sheep of iran. objectives: a molecular study was carried out in north khorasan province, iran in 2010-2011, designed to identify babesia spp. infection of both sheep and ticks. methods: ninety sheep from different flocks were clinically examined and blood samples were collected with ixodid  ticks . the collected ticks were separated into 82 ti...

background: chlamydia trachomatis (c. trachomatis) and mycoplasma genitalium (m. genitalium) are considered factors in cervical and ovarian cancer and are associated with flaky cell carcinoma of the cervix. the role of steady infection, leading to chronic inflammation, in the of ovarian cancer has received very little consideration, although a background of pelvic inflammatory disease (pid) is ...

a molecular survey was performed for identification theileria spp in sheep and ticks during from 2010-2011 in fasa and kazeroun areas, fars province, iran. a total of 100 sheep from different flocks were clinically examined and blood samples with ixodid ticks collected. the prepared blood smears from capillary vein of ear were stained with giemsa methods and examined by using light microscope. ...

background and objectives: the aim of this study was to compare direct microscopic examination with culture and pcr for the diagnosis of mucorales infection in blood and tissue specimens. material and methods: blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with suspected invasive fungal infection and cultured on proper media. direct smear of tissue samples ...

background: the aim of this study was to detect and characterize cryptosporidium spp. in water sam­ples collected from recreational ponds of chaharmahal va bakhtiyari province of iran . meth­ods: thirty water samples were collected  from november 2009 to may 2010. each sample con­tained 10 liters of water. we used the ssu rrna-based pcr-rflp technique. results: out of thirty samples examined, 6...

adenoviruses are recognized as common human pathogens that are responsible for a wide variety of infectious syndromes. bone marrow transplant patients are prone to life threatening opportunistic infections like adenoviruses. the nested polymerase chain reaction has provided an alternative, sensitive diagnostic method for detection of adenoviruses. in this study we developed pcr from hexon genes...