the 440 fertile eggs, in a completely randomized design were divided into 11 treatments with four replicatesand 10 eggs per each replicate. treatments including: (t1; control; without injection), (t2; injected with 0.3ml saline 9.0%; sham control), (t3, t4 and t5) 25, 75, 125 ppm fe-nano, (t6, t7 and t8) 50, 100, 150 ppmfe-nano-alimet chelate, (t9, t10 and t11) 50, 100, 150 ppm fe-aliment chela...