Arginine methylation constitutes a posttranslational modification dependent on the action of protein arginine methyltransferases (PRMTs). Using S-adenosylmethionine as a methyl donor, PRMTs catalyze the formation of monomethylarginine (L-NMMA), asymmetric dimethylarginine (ADMA), or symmetric dimethylarginine (SDMA). Protein arginine methylation is involved in the regulation of signal transduct...

Cutting-edge DNA methylation-based epigenetic aging techniques were applied to Gulf of Mexico northern red snapper (Lutjanus campechanus; n = 10; 1–26 years old) and grouper (Epinephelus morio; 2–14 old). Bisulfite-converted restriction site-associated sequencing was used identify CpG sites (cytosines followed by guanines) that exhibit age-correlated methylation, species-specific clocks develop...

The methylation of alcohols is great importance since a broad number bioactive and pharmaceutical contain methyl groups. Here, highly efficient ?-methylation primary secondary with methanol has been achieved by using bis-N-heterocyclic carbene iridium (bis-NHC-Ir) complexes. Broad substrate scope up to quantitative yields were at low catalyst loadings only hydrogen water as by-products. protoco...

Laminin-5 (LN5) anchors epithelial cells to the underlying basement membrane, and it is encoded by three distinct genes: LAMA3, LAMB3, and LAMC2. To metastasize and grow, cancer cells must invade and destroy the basement membrane. Our previous work has shown that epigenetic inactivation is a major mechanism of silencing LN5 genes in lung cancers. We extended our methylation studies to resected ...

The catalytic asymmetric methylation of fluoroalkylated pyruvates is shown with dimethylzinc as a methylating reagent in the presence of a copper catalyst bearing a chiral phosphine ligand. This is the first catalytic asymmetric methylation to synthesize various α-fluoroalkylated tertiary alcohols with CF3, CF2H, CF2Br, and n-C n F2n+1 (n = 2, 3, 8) groups in good-to-high yields and enantiosele...

Myelodysplastic syndromes (MDSs) are a clonal bone marrow (BM) disease characterized by ineffective hematopoiesis, dysplastic maturation and progression to acute myeloid leukemia (AML). Methylation silencing of HRK has been found in several human malignancies. In this study, we explored the association of HRK methylation status with its expression, clinical parameters and MDS subtypes in MDS pa...

Objective: This study aimed to investigate the association between global DNA and PTEN gene methylation changes type of delivery. Material Methods: The included a total 129 pregnant women at >37th week pregnancy who had cesarean (CS) (n=62) or vaginal delivery (VD) (n=67) their infant's cord blood (n=129). 5-mC ELISA method performed analyses, while change analyses were conducted using methylat...

Animal studies have linked perinatal bisphenol A (BPA) exposure to altered DNA methylation, but little attention is given to analyzing multiple physiologically relevant doses. Utilizing the viable yellow agouti (A(vy)) mouse, we examine the effects of developmental exposure through maternal diet to 50 ng BPA/kg (n = 14 litters), 50 μg BPA/kg (n = 9 litters), or 50 mg BPA/kg (n = 13 litters) on ...