A simple and convenient method has been developed for the determination of Dithiocarbamate fungicides e.g. Ziram, Thiram, Ferbam, Zineb and ZDC in technical form and in their formulations. Aliquots containing 1-5 mg of the sample were taken in a 100ml conical flask and 5ml of 0.02 N,N-Bromosuccinimide (NBS), 10ml of glacial acetic acid and 5ml of 4 N HCI were added to it. The contents were shak...

Modification of tryptophan side chains of soybean agglutinin (SBA) with N-bromosuccinimide results in a loss of the hemagglutinating and carbohydrate binding activities of the protein. One residue/subunit is probably essential for the binding activity. Modification leads to a large decrease in the fluorescence of the protein accompanied by a blue shift. Iodide ion quenching of the protein fluor...