I Introduction In recent years, specialists in human–computer interaction (HCI) have come to appreciate the importance of understanding the context in which computer-supported activities take place [1]. Such understanding directly affects design and evaluation by revealing what users are up to and how they might most effectively use a technology. The idea is to gain this understanding before th...

Standley, Paul R., Tamar J. Obards, and Cherie L. Martina. Cyclic stretch regulates autocrine IGF-I in vascular smooth muscle cells: implications in vascular hyperplasia. Am. J. Physiol. 276 (Endocrinol. Metab. 39): E697–E705, 1999.—Vascular smooth muscle cells (VSMC) subjected to acute or chronic stretch display enhanced growth rates in vitro and in vivo. Clinical examples of vascular hyperpla...

The present study was performed to evaluate the in vitro inhibitory potential of sarpogrelate and its active metabolite, M-1, on the activities of nine human cytochrome (CYP) isoforms. Using a cocktail assay, the effects of sarpogrelate on nine CYP isoforms and M-1 were measured by specific marker reactions in human liver microsomes. Sarpogrelate potently and selectively inhibited CYP2D6mediate...

Here we report the development of a gene-synthesis technology, circular assembly amplification. In this approach, we first constructed exonuclease-resistant circular DNA via simultaneous ligation of oligonucleotides. Exonucleaseand subsequent mismatch cleaving endonuclease–mediated degradation of the resulting ligation mixture eliminated error-rich products, thereby substantially improving gene...

JAMES R. GUM, JR.,1,2 JAMES W. HICKS,3 ANNE-MARIE GILLESPIE,4 ELAINE J. CARLSON,4 LAZLO KÖMÜVES,5 SATYAJIT KARNIK,1 JOE C. HONG,3 CHARLES J. EPSTEIN,4 AND YOUNG S. KIM1,3 1Gastrointestinal Research Laboratory, Department of Veterans Affairs Medical Center, San Francisco 94121; and Departments of 2Anatomy, 3Medicine, 4Pediatrics, and 5Dermatology, University of California at San Francisco, Calif...

The present study was performed to evaluate the in vitro inhibitory potential of sarpogrelate and its active metabolite, M-1, on the activities of nine human cytochrome (CYP) isoforms. Using a cocktail assay, the effects of sarpogrelate on nine CYP isoforms and M-1 were measured by specific marker reactions in human liver microsomes. Sarpogrelate potently and selectively inhibited CYP2D6mediate...

Schemmer, Peter, Blair U. Bradford, Michelle L. Rose, Hartwig Bunzendahl, James A. Raleigh, John J. Lemasters, and Ronald G. Thurman. Intravenous glycine improves survival in rat liver transplantation. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G924–G932, 1999.—In situ manipulation by touching, retracting, and moving liver lobes gently during harvest dramatically reduces survival af...

Wetter, Thomas J., Annie C. Gazdag, David J. Dean, and Gregory D. Cartee. Effect of calorie restriction on in vivo glucose metabolism by individual tissues in rats. Am. J. Physiol. 276 (Endocrinol. Metab. 39): E728–E738, 1999.—We evaluated the effects of 8 mo of calorie restriction [CR: 60% of ad libitum (AL) food intake] on glucose uptake by 14 tissues in unanesthetized, adult (12 mo) F3443BN ...

Darimont, Christian, Nathalie Gradoux, and Alain De Pover. Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G606–G612, 1999.—Epidermal growth factor (EGF) has been reported to stimulate carbohydrate, amino acid, and electrolyte transport in the small intestine, but its effects on lipid transport are poorly...