The principal objective of this study was to derive an improved procedure for cryopreservation of swamp buffalo (Bubalus bubalis) spermatozoa. Experiments were conducted to determine effects of cooling rate, intermediate plunge temperature and warming rate on motility and acrosome integrity of spermatozoa. Spermatozoa were obtained from three bulls (three ejaculates/bull) and were subjected to ...

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AIM The aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (CLC) treated buffalo spermatozoa. MATERIALS AND METHODS Semen samples with mass motility of 3+ and greater, collected from Murrah buffalo bulls were utilized. Immediately after collection, four equal groups of semen sample were made. Group I was kept as control and diluted...

In order to contribute to the breeding programmes of Asian water buffalo, the aim of this study was to analyse the influence of genetic effects in the stayability of Murrah dairy buffaloes. The stayability trait (ST) was defined as the female's ability to stay in the herd for one (ST1), two (ST2), three (ST3), four (ST4), five (ST5) or six years (ST6) after the first calving. The same trait was...

AIM To test the hypothesis that season affects the semen quality of swamp buffalo (Bubalus bubalis) bulls used for artificial insemination (AI) under tropical conditions in Thailand, as it does in Bos taurus and Bos indicus. METHODS Clinical and andrological examinations, and monitoring of semen production and quality were carried out on five mature, healthy swamp buffalo AI bulls in Thailand...

The functional integrity of spermatozoa in various domestic animals like horse, cattle and swine is evaluated by hypo osmotic swelling test. The hypo osmotic swelling test has not been tested in the fresh semen of Nili-Ravi buffalo bull. The aim of the present study was evaluation of membrane integrity by establishing the best hypo osmotic swelling solution. Ten solutions with different osmolar...