“Heat-aid kit” is a rapid detection kit which was developed by the application of ELISA. This qualitative test was indicated by colour change i.e based on the presence of progesterone. The present research was carried out to evaluate the accuracy of heat aid kit, a rapid detection test for early pregnancy diagnosis in graded Murrah buffaloes. For this, the milk samples were collected from 16 bu...

The current experiment was conducted to reduce the cost of superovulation by reducing the dose of FSH by partial replacement with PMSG. Five elite, multiparous, Murrah buffaloes, 80 to 120 days postpartum, were included in multiple-ovulation embryo transfer (MOET) schedule. The estrus was synchronized with one injection of prostaglandin. Superovulatory treatment was started from day 10 of induc...

Present study was undertaken to compare the effect of two different milking methods (hand vs. machine milking) on milking ability in Murrah buffaloes and Sahiwal cows. 30 lactating Sahiwal cows and 30 lactating Murrah Buffaloes were selected and divided into four equal groups having 15 animals each. 15 animals of each species were subjected to machine milking and 15 animals of each species were...

Caffeine was incorporated in egg yolk tris glycerol extender (EYTG) at the time of dilution of Murrah semen at a concentration of 1.0, 3.0 or 5.0 mM. The samples were subsequently frozen. The post thaw progressive motility and live sperm per cent improved signifi cantly (P<0.05) with addition of 1.0 (70.0±0.99 and 88.80±0.84) and 3.0 mΜ (65.83±0.88 and 86.16±0.96%) caffeine to the extender befo...

Materials and Methods Dietary supplementation of probiotic yeast ( ) was noted to improve the animal performance by improving the digestibility of nutrients and rumen bacterial number [1]. Addition of yeast culture, as a probiotic, in animal feeds improves fibre digestion [2], the cellulolytic activity of rumen microbes [3], and reduces the lactate accumulation and oxygen concentration in rumen...

BACKGROUND Heat stress adversely affects the physiological and metabolic status, and the productive performance of buffalo. METHODS The present study was conducted to explicate the effect of misting and wallowing cooling strategies during heat stress in lactating Murrah buffalo. The study was conducted for three months (May-July) of which first two months were hot dry and last month was hot h...

Background: This study examined the effect of including one of two growth factors (100 ng/ml IGF-1 or 20 ng/ ml EGF) in combination with one of two antioxidants (50 μM cysteamine or 50 μM beta-mercaptoethanol) in invitro maturation of buffalo oocytes, followed by fertilization and subsequent development of embryos. Materials and Methods: The oocytes were recovered by in vivo ovum pick-up techni...

Background: Marker assisted selection based strategies are essential to identify superior quality bulls at an early age for frozen semen production. The present study aims determine the genetic variants in Y-chromosome specific genes, sex determining region on (SRY) and testis protein Y-encoded (TSPY) association with production traits Murrah buffalo bulls. Methods: genomic DNA extracted from b...