OBJECTIVES The global occurrence of antibiotic resistance genes in bacteria in water environments is an increasing concern. Treated wastewater was sampled daily over a 45 day period from the outflow of a municipal wastewater treatment plant in Brno, Czech Republic, and examined for extended-spectrum β-lactamase (ESBL)-producing bacteria. METHODS Water samples were cultivated on MacConkey agar...

Shiga toxin-producing Escherichia coli strains are human pathogens linked to hemorrhagic colitis and hemolytic uremic syndrome. The major virulence factors of these strains are Shiga toxins Stx1 and Stx2. The majority of the genes coding for these toxins are borne by bacteriophages. Free Stx2-encoding bacteriophages have been found in aquatic environments, but there is limited information about...

WARREN H. CHESNER Chesner Engineering, P.C. This paper presents an overview of a recently pat­ ented technology which defines the process of using selected waste products to assist in reclaiming existing municipal solid waste landfill sites [1]. The process in­ volves the pressurized injection of these materials into the void spaces of existing landfill sites to both manage the waste product an...

Plasmids pRSB113 and pRSB115 were recovered from an activated sludge bacterial community of a municipal wastewater treatment plant in Germany. Both plasmids carry the same bla(GES-5) carbapenemase gene, located within two distinct class 1 integrons. These plasmids have different backbones, belong to different incompatibility groups, and could replicate in both Pseudomonas aeruginosa and Escheri...

This paper addresses the question of estimating unknown inputs in biological processes. The unknown inputs are either constant or periodic (as e.g. in municipal wastewater treatment plants where the loading rate is unknown yet of a period equal to one day). In particular the designed observer is able to recover the unknown periodic inputs over a few periods.

Bacteriophage OSYSP is a new anti-Escherichia coli O157:H7 phage isolated from municipal wastewater in Ohio. OSYSP is potent against enterohemorrhagic E. coli and is a candidate biocontrol agent for food and therapeutic applications. In this paper, we present the important genetic features of this phage based on its complete genome sequence.

Enterobacter cloacae strain JD6301 was isolated from a mixed culture with wastewater collected from a municipal treatment facility and oleaginous microorganisms. A draft genome sequence of this organism indicates that it has a genome size of 4,772,910 bp, an average G+C content of 53%, and 4,509 protein-coding genes.

The average American generates approximately 4.5 pounds of garbage per day, for a total of 196 million tons of trash per year, most of which ends up in landfills (Shortridge, 1993). Various government and private programs have been organized to divert part of this garbage (metals, plastics, paper and cardboard, and yard and food wastes) from landfills by recycling. Yard and food wastes often ar...

Here, we report the complete genome sequence of the triclosan- and multidrug-resistant Pseudomonas aeruginosa strain B10W, obtained from municipal wastewater in Hawaii. The bacterium has a 6.7-Mb genome, contains 6,391 coding sequences and 78 RNAs, with an average G+C content of 66.2 mol%.

The paper is a short review on nitrogen compounds removal, as ammonia, nitrate, nitrite, from municipal waste water, containing primary information of nitrogen chemistry, consideration on the physical-chemistry methods of removal and also on the most popular wastewater nitrification systems[1].