The partitioning of the n-alkyl-p-aminobenzoates into the lipid bilayer is dependent not only on their physicochemical properties, but also on temperature and bilayer composition. Partitioning studies with this homologous series demonstrate the effect of alkyl chain length on partitioning in pure DPPC liposomes at 23 and 50°C and in DPPC : Chol liposomes at 23°C. A general trend is observed whe...

In spite of its widespread use, benznidazole's (BNZ) toxicity and low efficacy remains as major drawbacks that impair successful treatments against Chagas disease. Previously, attempting to increase the selectivity and reduce its toxicity on infected tissues, multilamellar liposomes (MLV) composed of hydrogenated soybean phosphatidylcholine (HSPC): distearoyl-phosphatidylglycerol (DSPG): choles...

A comparison is made of the antioxidant activity of a water-soluble form of alpha-tocopherol complexed with bovine serum albumin (alpha-T X BSA) with that of micellar alpha-tocopherol and aqueous 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylate (Trolox) to inhibit autoxidation of linoleic acid in sodium dodecyl sulfate micelles. The peroxyl radical trapping ability of alpha-T X BSA compares f...

Peri, Irena, Hanna Mamrud-Brains, Sergey Rodin, Valery Krizhanovsky, Yechiel Shai, Shlomo Nir, and Michael Naim. Rapid entry of bitter and sweet tastants into liposomes and taste cells: implications for signal transduction. Am. J. Physiol. Cell Physiol. 278: C17–C25, 2000.— Some amphipathic bitter tastants and non-sugar sweeteners are direct activators of G proteins and stimulate transduction p...

The purpose of this study was to find a multilamellar liposomal formulation for the antichagasic drug Benznidazole (BNZ). Different lipid matrices and organic solvents for BNZ were tested in order to obtain the liposomes with the highest g BNZ/100 g total lipid (D/TL) ratio. The best lipid matrices resulted from hydrogenated phosphatidylcholine from soybean (HSPC): Cholesterol (Chol): distearoy...

The potency of nanoparticles, loaded with drugs, genes, and/or diagnostics, is frequently depending upon their morphology adopted in a biological relevant environment. Freeze-fracture electron microscopy is a powerful techniques to monitor the self-assembling of lipid-, polymer-, as well as protein/peptide-based drug and gene carries on a nano-size resolution scale. Freeze-fracture electron mic...

We have previously reported a new preparation method for liposomes using supercritical carbon dioxide (scCO2) as a solvent, referred to as the supercritical carbon dioxide reverse phase evaporation (scRPE) method. In our previous work, addition of ethanol to scCO2 as a co-solvent was needed, because lipid molecules had to be dissolved in scCO2 to form liposomes. In this new study, niosomes (non...

Objective To determine and compare the SPF (Sun Protection Factor) and moisturizing effects of the liposomal and conventional lotion formulations containing octyl methoxycinnamte (OMC) as a sunscreen by in vivo methods. Materials and Methods The multilamellar liposomes (MLVs) containing OMC were prepared by fusion method and o/w emulsion was prepared as FDA standard sunscreen method. The SPF...

BACKGROUND The contribution of fibrinogen (FBN) to hemostasis acting on platelet aggregation and clot formation is well established. It has been suggested that FBN-coated liposomes could be useful in restoring hemostasis. In the present study, we evaluated the modifications induced by multilamellar raw liposomes (MLV) or fibrinogen-coated liposomes (MLV-FBN) on hemostatic parameters. MATERIAL...

PURPOSE To elucidate the effect of physicochemical properties of nanocarrier systems on drug delivery efficiency to the retina by eyedrop administration in mice, rabbits, and monkeys. METHODS Submicron-sized liposomes (ssLips) of different particle size, cholesterol content, surface charge, and multilamellar vesicles (MLV) were prepared by the hydration METHOD Fluorescence probe (coumarin-6...