Medium from mouse mammary tumor cell cultures con tained a growth-stimulating factor. The assay for growthstimulating factor was performed on density-inhibited cul tures of mouse embryo cells. Addition of nondialyzed or dialyzed mouse mammary tumor cell culture medium caused an increase in DNA synthesis and mitosis in densityinhibited cells. Dialyzed medium was more active than non dialyzed med...

We have demonstrated compression stress induced mechanical deformation of microtubules (MTs) on a two-dimensional elastic medium and investigated the role of compression strain, strain rate, and a MT-associated protein in the deformation of MTs. We show that MTs, supported on a two-dimensional substrate by a MT-associated protein kinesin, undergo buckling when they are subjected to compression ...

Metallothioneins (MTs) are cysteine-rich proteins required for heavy metal tolerance in animals and fungi. Two cDNAs encoding proteins with homology to animal and fungal MTs have been isolated from Arabidopsis. The genes represented by these cDNAs are referred to as MT1 and MT2. When expressed in an MT-deficient (cup1 delta) mutant of yeast, both MT1 and MT2 complemented the cup1 delta mutation...

Background: The aim of the present study was to develop a three-dimensional (3D) collagen gel culture system for the in vitro growth and survival of buffalo preantral follicles with or without growth factors. Materials and Methods: Buffalo ovaries were collected from a local abattoir and preantral follicles were isolated through microdissection. Isolated preantral follicles were put either in c...

sugar beetis an essentially self-sterile plant for which the vegetative propagation is used to obtain identical sister plants. sugar beet micropropagation is initiated by meristematic cuttings. sugar beet meristematic flower stalk explants were established in sterile cultures and used for shoot propagation. two pgob medium with different hormonal combinations: 1) ba/naa/iba at 1, 0.1 and 0.1 an...

Abstract The experiments were conducted in the Laboratory of Tissue culture/Department Forestry/College Agriculture and Forestry/the University Mosul. To propagate three citrus rootstocks (Citrus aurantium L., Citrumelo swingle , Cariso 35 (C35))In vitro . During Initiation stage, single nodes planted on a solid MS medium supplemented with five concentrations BA (0, 1, 2, 3, 4 mg L -1 ). After ...

this study was designed to examine the effects of retinoic acid adding to cumulus and/or fibroblast cellsmonolayer on the development of mouse early embryos. one-cell mouse embryos were obtained from nmrimice after superovulation by an intraperitoneal injection of 5 iu equine chorionic gonadotrophin (ecg)followed 48 hrs later by 5 iu human chorionic gonadotrophin (hcg). mouse embryonic fibrobla...

Background: Bacterial Extracellular Polymeric Substances (EPS) are environmental friendly and versatile polymeric materials that are used in a wide range of industries such as: food, textile, cosmetics, and pharmaceuticals. To make the production process of the EPS cost-effective, improvements in the production yield is required which could be implemented through application of processes such a...

BACKGROUND AND OBJECTIVES Tuberculosis (TB) is a major problem in the world. Treatment and control of TB needs detection of the Mycobacterium tuberculosis (MT) in the proper samples. While smear doesn't have enough sensitivity, culture and PCR are expensive, time consuming and unavailable in many centers. Recent development of a rapid TB antigen detection test (PrTBK) at Pasteur Institute of Ir...