On 2020 November 30, Parker Solar Probe (PSP) was crossed by a coronal mass ejection (CME)-driven shock, which we suggest also crossing convected, isolated magnetic structure (MS) at about the same time. By analyzing PSP/FIELDS field measurements, find that leading edge of MS coincided with while its trailing edge, identified as current sheet, overtook PSP 7 minutes later. Prior to arrival flux...

There are tow major methods that are widely used for protein identification by mass spectrometry: MALDI-TOF based protein fingerprinting and LC-MS/MS based peptide sequencing. In the MALDI-TOF based protein fingerprinting method, a sample is digested with certain proteolytic enzyme (usually trypsin) and one MS spectrum is acquired which generates the massed of all peptides (or MH+), and these m...

Citrus-type crude drugs (CCDs) are commonly used to formulate decoctions in Kampo formula (traditional Japanese medicine). Our previous study reported metabolomic analyses for differentiation of the methanol extracts using ultra-HPLC (UHPLC)/MS, and 13C- 1H-NMR. The present expanded scope its application by analyzing four CCD water (Kijitsu, Tohi, Chimpi, Kippi); these CCDs usually as decoction...

There are well over 500 registered pesticides worldwide for use in agricultural regions and new agrochemicals are introduced to the marketplace continuously. This chapter deals with the chemical analysis methods for the main pesticide chemical classes that are most frequently analyzed with gas chromatography (GC) or liquid chromatography (LC) coupled to mass spectrometry (MS). GC amenable pesti...

Analyzing hair for many substances can be tedious and expensive, and a rapid screening method should prove helpful. Generally, screening has been performed using immunological tests, mainly in workplace drug testing, where the number of samples has been high. The aim of this study was to develop an LC-MS-MS method for the simultaneous analysis of several drugs of abuse in human hair as an alter...

BACKGROUND Myocardial T1 and T2 mapping using cardiovascular magnetic resonance (CMR) are promising to improve tissue characterization and early disease detection. This study aimed at analyzing the feasibility of T1 and T2 mapping at 3 T and providing reference values. METHODS Sixty healthy volunteers (30 males/females, each 20 from 20-39 years, 40-59 years, 60-80 years) underwent left-ventri...

The thesis describes the development of new and improved methods for analyzing degradation markers from organophosphorus Chemical Warfare Agents (CWAs). Paper I and II describes an innovative and significantly improved method for the enrichment, derivatization (trimethysilylation) and GC-MS analysis of a broad range of organophosphorus CWAs degradation markers, namely the alkylphosphonic acids ...

Affinity purification followed by mass spectrometry (AP-MS) has become a common approach for identifying protein-protein interactions (PPIs) and complexes. However, data analysis and visualization often rely on generic approaches that do not take advantage of the quantitative nature of AP-MS. We present a novel computational method, nested clustering, for biclustering of label-free quantitative...

Molecular mechanisms responsible for acute relapse of multiple sclerosis (MS) remain currently unknown. The aim of this study is to identify the relapse-specific biomarker genes in T lymphocytes of relapsing-remitting MS (RRMS). Total RNA of CD3+ T cells isolated from six RRMS patients taken at the peak of acute relapse and at the point of complete remission was processed for DNA microarray ana...

Protein phosphorylation catalyzed by kinases plays crucial roles in regulating a variety of intracellular processes. Owing to an increasing number of in vivo phosphorylation sites that have been identified by mass spectrometry (MS)-based proteomics, the RegPhos, available online at http://csb.cse.yzu.edu.tw/RegPhos2/, was developed to explore protein phosphorylation networks in human. In this u...