Wax-coated capsules containing selective ingredients (brilliant green and oxgall) were added at the time of inoculation of most-probable-number media (modified lactose broths). The inhibitory ingredients gradually diffused from the capsules into the nonselective media, imparting selectivity to the media. Concentrations of brilliant green did not reach inhibitory levels until 2 or more h had ela...

Objective The objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. Methods Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These sam...

Air has been established as a source of bacterial contamination in meat processing facilities. Airborne bacteria may affect product shelf life, and have food safety implications. The effectiveness of reactive oxygen species (ROS) generating AirOcare equipment on the reduction of airborne bacteria in a meat processing environment was determined. Bacterial strains found in ground beef were used t...

Different tests for the identification of Gardnerella (Haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. A presumptive identification of G. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta...

A new differential and selective medium, DYS agar, was developed and evaluated from the isolation of Yersinia enterocolitica. Ther bile salts content of the medium resulted in high selectivity, and inclusion of arabinose, lysine, and arginine rendered Y. Enterocolitica very distinct from Proteus spp., Pseudomonas spp., and other members of the family Enterobacteriaceae. DYS medium was more effi...

The antagonistic effect of lactic acid bacteria (LAB, including Lactobacillus acidophilus, L. bulgaricus, L. casei and Streptococcus thermophilus) on Staphylococcus aureus and Escherichia coli was evaluated on MRS agar with the deferred and cross-streaking techniques, and in milk with the plate counting method. All LAB were repressive to S aureus and E coli on the agar medium. However, their su...

A healthy vaginal ecosystem has been shown to be protective against the acquisition of human immunodeficiency virus and gonorrhea, and women who are colonized with H(2)O(2)-producing lactobacilli are more likely to maintain a normal vaginal flora than women with lactobacilli that do not produce H(2)O(2). The purpose of this study was to formulate a testing medium that better supports the growth...

This research aim to preliminarily investigate biofilm production among Vibrio species isolated from Siloso beach, Singapore. 100 μL of sea water sample collected was plated on Congo Red Agar (CRA) using spread plate method. The plates were incubated at 30°C for 24 hours. Forty – six biofilm producing black colonies with dry crystalline consistence were further transferred aseptically into fres...

In rat serum as well as in human serum and bile after injection of cefotaxime (CTX), the parent compound and the active metabolite desacetyl cefotaxime (dCTX) have been demonstrated by quantitative analysis with high-performance liquid chromatography (HPLC). Simultaneous determination of CTX by bioassay using test organisms sensitive to both CTX and dCTX resulted in spuriously high concentratio...