Short (15 min) and long (1 hour or more) pulses with P32 have been compared at different developmental stages of Limnaea. In short pulses, as opposed to long-pulses, the relative percentage of 4S RNA is at a more or less constant level at all stages of development. Heavy RNA, i. e. heavier than 28S, is traceable with both short and long pulses in the uncleaved and early morula stages. Transcrip...

Abstract Study question Screen the key gene groups that determine development of blastocyst. Explore effect ovol1/2 on early embryonic development. Summary answer We founded there were insufficient activation Major ZGA genes in AE group,and focused candidate ovol1/2. What is known already At present, clinical success rate assisted reproductive technology (ART) less than 50%. A large number embr...

Ultrastructure of the mouse morula at the time of formation of the outer cell layer is described. Analysis of relative positions of sister blastomeres, still connected with cytoplasmic bridge, suggests that cell division may occur both tangentially and perpendicularly in relation to the embryo surface. However, there are observations suggesting that active cell movement may be involved in cell ...

in order to do in vitro fertilization (ivf), 435 oocytes were collected from 20 super ovulated nmri female mice, in m2 media with 4% bsa. 85% (n=370) of collected oocytes were in mii phase. epididymal sperms were added to 332 oocytes in t6 media drops with 4% bsa and incubated. fertilization rate was 79.8% (n=265) out of which 27.5% (n=73) grew to morula and 20.8% (n=55) achieved blastocyst sta...

Preimplantation genetic diagnosis (PGD) is commonly performed on biopsies from 6-8-cell-stage embryos or blastocyst trophectoderm obtained on day 3 or 5, respectively. Day 4 human embryos at the morula stage were successfully biopsied. Biopsy was performed on 709 morulae from 215 ICSI cycles with preimplantation genetic screening (PGS), and 3-7 cells were obtained from each embryo. The most com...

Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. MaterialsAndMethods Bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. Their presumptive...