The cell-cell interactions between fibroblasts and mononuclear leukocytes (MNL) which promote alterations in collagen accumulation were examined using a system of co-culture of human fibroblasts and peripheral blood MNL. The stimulation of collagen production was optimal after 48 h of co-culture and the increase in collagen correlated directly with the number of MNL added. The enhancement of co...

Conventional (cv) and germfree (gf) mice are able to give a good proliferative response to allogeneic cells in the mixed leukocyte culture (MLC) test, while the response to xenogeneic stimulating cells has been in question. Previous studies by others have suggested only a low MLC response in cv animals and none in gf ones. We have found that both cv and gf animals can give a good MLC response t...

The mixed lymphocyte culture (MLC) test has been regarded as an in vitro model of the recognition or sensitization phase of the homograft or graft-versus-host reaction. It has been suggested that the graft-versus-host response in vivo is less in xenogeneic combinations than in allogeneic ones and that there is a similar quantitative relationship in MLC responses. Given the above interpretation ...

Blood leukocytes from an immunologically hyporesponsive patient with urinary bladder carcinoma were found to be deficient in their ability to stimulate 3 of 27 responder leukocyte preparations from normal individuals in one-way mixed leukocyte culture (MLC). The patient's T-depleted leukocytes, however, functioned adequately as stimulator cells. T-enriched lymphocytes from this patient suppress...