Sequence analysis of peptide deformylase of Mycobacterium tuberculosis revealed the presence of insertions (residues 74 to 85) and an unusually long carboxy-terminal end (residues 182 to 197). Our results with deletion mutants indicated the contribution of these regions in maintaining enzymatic stability. Furthermore, we showed that the region spanning the insertions was responsible for maintai...

Hybrid nanocomposites of binary Pt-Ru/Polyaniline were prepared by oxidative polymerization of aniline andformation Pt and Ru nanoparticles. The polymerization of aniline was carried out in the presence of Potassiumhexa cyano Platinate (IV) and Ruthenium (III) nitrosyl nitrate as oxidizing agents. During the reaction anilinemonomers undergo oxidation and form polyaniline (PANi) whereas the redu...

Three aryl methyl sulfoxides were prepared via the following sequence: synthesis of menthyl arenesulfenate from corresponding thiol, diastereoselective oxidation leading to sulfinate, then reaction with a Grignard reagent. The moiety was substituted ester on ortho position or nitro group para position. sulfenate esters obtained in fair excellent yield (42–82%). Four different oxidizing agents t...

We investigated six endodontic agents for their ability to induce apoptosis and modify the cytotoxic activity of NaF against human squamous cell carcinoma (HSC-2) and human promyelocytic leukemia (HL-60) cell lines. Four Group I agents (Form Cresol, Cam Phenic, Eucaly Soft, GC Fuji Varnish), but not two Group II agents (Caviton, Canals-N), induced internucleosomal DNA fragmentation and activate...

Designed polypeptides and electrostatic layer-by-layer self-assembly form the basis of promising research in bionanotechnology and medicine on development of polyelectrolyte multilayer films (PEMs). We show that PEMs can be formed from oppositely charged 32mers containing several cysteine residues. The polypeptides in PEMs become cross-linked under mild oxidizing conditions. This mimicking of d...

We previously reported that if murine leukemia virus particles are produced in the presence of the mild oxidizing agent disulfide-substituted benzamide-2, they fail to undergo the normal process of virus maturation. We now show that treatment of these immature particles with a reducing agent (dithiothreitol) induces their maturation in vitro, as evidenced by proteolytic cleavage of Gag, Gag-Pol...

A bifunctional nanocatalyst composed of iron containing SBA-15 material modified with sulfonic acid groups was synthesized by a mechanochemical approach. A full characterization of the obtained nanocatalyst was performed by N2 physisorption isotherms analysis, transmission electron microscopy (TEM), X-ray powder diffraction (XRD) and Fourier-Infrared Spectroscopy (FT-IR). The mechanochemically ...

PURPOSE To identify the mechanisms by which oxidative stress with oxidizing agents alters the activity of ion channels in human retinal pigment epithelial (RPE) cells. METHODS The effects of oxidizing agents on ion currents were investigated in human RPE R-50 cells with the aid of the whole-cell, cell-attached, and inside-out configurations of the patch-clamp technique. RESULTS In the whole...

textile wastewaters due to the toxic effects of dyestuffs and other organic compounds and their stability toward light and oxidizing agents have led to an environmental problem. several treatment methods for dye removal have been investigated. membrane process is one of the simplest and most effective methods for dye removal from industrial wastewaters. electrospun nanofibrous membranes have hi...