BACKGROUND To understand if relapse, following antimicrobial treatment was due to re-infection or to recrudescence. METHODS Fifty patients with dyspepsia were studied prospectively. They were followed up by endoscopy and biopsy of antral mucosa before and after treatment with anti-microbial therapy. Gel isoelectrofocusing was used to characterize protein profile of Hp. RESULTS At baseline 4...

ABSTRACT- Despite the benefits of herbicides, their persistence in soil can harm other plants in next rotations and contaminate ground water. The main purpose of this research is to simulate 2,4-dichlorophenoxyaceticacid(2,4-D) movement in silty clay loam soil using HYDRUS-1D model in a corn root zone. The maximum contaminant level goal, MCLG, of 2,4-D in water resources is 10 ppb. The 3.5 kg a...

There is currently much interest in the interactions between exotic plants and soil organisms. Exotic invasive species can have profound effects on the microbial community of the soil and positive feedback of soil biota to invasive plants may facilitate their successful invasion. To better understand the impacts of Ambrosia artemisiifolia L. invasion on microbial carbon source utilization and r...

The most important single factor affecting the efficiency of protein utilization is the profile of digestible essential amino acids entering the small intestine. Assuming a constant ideal amino acid profile of absorbed protein the requirements of all amino acids can be calculated when the requirement of one individual amino acid has been determined. In non-ruminants, the supply will be greatly ...

The conversion of virgin desert into oasis farmland creates two contrasting types of land-cover. During oasis formation with irrigation and fertilizer application, however, the changes in the soil microbial population, which play critical roles in the ecosystem, remain poorly understood. We applied high-throughput pyrosequencing to investigate bacterial and archaeal communities throughout the p...

BACKGROUND Increasing evidence supports the central role of Paneth cells in maintaining intestinal host-microbial homeostasis. However, the direct impact of host genotype on Paneth cell function remains unclear. Here, we characterize key differences in Paneth cell function and intestinal microbial composition in two widely utilized, genetically distinct mouse strains (C57BL/6 and 129/SvEv). In ...

The direct "metagenomic" sequencing of genomic material from complex assemblages of bacteria, archaea, viruses and microeukaryotes has yielded new insights into the structure of microbial communities. For example, analysis of metagenomic data has revealed the existence of previously unknown microbial taxa whose spatial distributions are limited by environmental conditions, ecological competitio...

Human gastrointestinal microbial communities are recognized as important determinants of the host health and disease status. We have recently examined the distal gut microbiota of two groups of children: healthy adolescents and those diagnosed with diarrhea-predominant irritable bowel syndrome (IBS). We have revealed the common core of phylotypes shared among all children, identified genera dif...

A field experiment was conducted to illustrate the different degree and dynamics of microbial community structure and function in the rhizosphere across four growing stages (before plantation and three growth stages) using a combination of biochemical (enzyme assay and microbial biomass carbon) and molecular approaches of qPCR and PCR-DGGE (polymerase chain reaction-denaturing gradient gel elec...

A method for analysis of volatile organic compounds (VOCs) from microbial cultures was established using proton transfer reaction-mass spectrometry (PTR-MS). A newly developed sampling system was coupled to a PTR-MS instrument to allow on-line monitoring of VOCs in the dynamic headspaces of microbial cultures. The novel PTR-MS method was evaluated for four reference organisms: Escherichia coli,...