A biodegradable polyester resin was polymerized from N-benzyloxycarbonylL -glutamic acid and ethylene glycol. Rhizopus delemar lipase was used as a biocatalyst for the rupture of ester bonds during the hydrolysis studies. Depolymerization was observed to followa Michaelis–Menten mechanism, with the maximumrate of monomer formation dP/dtmax = 1.12 x 10-8 mol/s and the rate constant Km x 2.03 x 1...

The authors generalise the concept of the geometric phase in stochastic kinetics to a non-cyclic evolution. Its application is demonstrated on kinetics of the Michaelis-Menten reaction. It is shown that the non-periodic geometric phase is responsible for the correction to the Michaelis-Menten law when parameters, such as a substrate concentration, are changing with time. The authors apply these...

Jacobsen CF, Leonis J, Linderstr0m-Lang K, Ottsen M. The pH-stat and its use in biochemistry. Methods Biochem Anal 1957;4:171-210. 2 Heller M, Hanahan DJ. Human erythrocyte membrane bound enzyme: acetylcholinesterase. Biochim Biophys Acta 1972;255:251-72. 3 Newman PFJ. A study of acetylcholine acetyl-hydrolase in the human erythrocyte. MPhil thesis, Edinburgh University, 1974. 4 Baggott J. The ...

The use of enzymes to catalyze chemical reactions has increased these recent years. Several models have been developed express the kinetics over biocatalysts. most well-known them, Michaelis-Menten, is used when only one substrate adsorbs on enzyme. In case esterification reaction, i.e., bimolecular system, a more complex kinetic model such as Ping-Pong Bi-Bi should be applied. advanced essenti...

The reactivity of tyrosinase adsorbed on nanogold bound with 4,4'-bis(methanethiol)biphenyl monolayer self-assembled on a gold disk with catechol in a dipolar aprotic solvent, acetonitrile (AN), was studied by cyclic voltammetric and amperometric methods. Tyrosinase exhibited characteristics of a Michaelis-Menten kinetic mechanism. The tyrosinase attached to the nanogold continued to react with...