Micellar electrokinetic chromatography (MEKC) was developed as a method for quantitative determination of gibberellic acid (GA3) in fermentation broth and commercial products, using 25 mM disodium tetraborate as a buffer at pH 9.2 and 100 mM sodium dodecyl sulfate as a micellar phase. The baseline resolution (Rs of GA3 from other compounds in fermentation broth was achieved with Rs > 2.5. The a...

The electrophoretic separation of electrically neutral benzodiazepines (BZDs) can be achieved by micellar electrokinetic chromatograpy (MEKC). The cyclodextrin (CD)-modified micellar electrokinetic chromatography separation of eight of the most frequently used BZD was obtained within 15 minutes at + 25 kV on a 64.5 cm x 50 μm I.D. silica capillary at 25C, using a 25 mM sodium tetraborate-50 mM ...

The principal advantage of the use of Shopl'skii effect (low temperature spectrum) is that spectral sharpening occurs both in absorption and emission. However, thus far using the technique of capillary electrophoresis/low temperature fluorescence spectroscopy (CE/LTFS) either at 77 or 4.2 K remains difficult to obtain an on-line spectrum, if the analyte is present at low concentration. This pap...

A sweeping technique, in conjunction with micellar electrokinetic chromatography, for the simultaneous determination of flunitrazepam and its major metabolites, 7-aminoflunitrazepam and N-desmethylflunitrazepam, is described. The optimized conditions for the sweeping and separation were a pH 9.5 buffer, 25mM borate, 50mM cetyltrimethylammonium bromide, 30% MeOH (v/v), and a 151-mm injection len...

Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed, namely; reverse micellar system, aqueous two phase...

objective(s): dispersive liquid-liquid microextraction coupled with gas chromatography (gc)-flame ionization detector was developed for the determination of valproic acid (vpa) in human plasma. materials and methods: using a syringe, a mixture of suitable extraction solvent (40 µl chloroform) and disperser (1 ml acetone) was quickly added to 10 ml of diluted plasma sample containing vpa (ph, 1.0...

Polarity Reversed-phase chromatography and hydrophobic-interaction chromatography Centrifugation: Analytical Centrifugation. Chromatography: Protein Separation. III/Enzymes: Liquid Chromatography. Proteins: Capillary Electrophoresis; Centrifugation; Crystallization; Electrophoresis; Glycoproteins: Liquid Chromatography; High-Speed Countercurrent Chromatography; Ion Exchange; Metalloproteins: Ch...

Silica mesoporous crystals (SMCs) offer a unique opportunity to study micellar mesophases. Replication of non-equilibrium mesophases into porous silica structures allows the characterization of surfactant phases under a variety of chemical and physical perturbations, through methods not typically accessible to liquid crystal chemists. A poignant example is the use of electron microscopy and cry...