Immobilization of enzymes on some solid supports has been used to stabilize enzymes in organic solvents. In this study, we evaluated applications of genetically immobilized Rhizopus oryzae lipase displayed on the cell surface of Saccharomyces cerevisiae in organic solvents and measured the catalytic activity of the displayed enzyme as a fusion protein with alpha-agglutinin. Compared to the acti...

The objective of this study was to determine the contribution of myocardial triglycerides to overall ATP production in isolated working rat hearts. Endogenous lipid pools were initially prelabeled (pulsed) by perfusing hearts for 60 min with Krebs-Henseleit buffer containing 1.2 mM [1-14C]palmitate. During a subsequent 60-min period (chase), hearts were perfused with either no fat, low fat (0.4...

The aim of this study was to investigate the effect of increasing exogenous palmitate concentration on carbohydrate and palmitate oxidation in hearts from control and 1-wk diabetic rats. Hearts were perfused with glucose, [3-13C]lactate, and [U-13C]palmitate. Substrate oxidation rates were determined by combining13C-NMR glutamate isotopomer analysis of tissue extracts with measurements of oxyge...

Investigation of the chemical composition of the essential oils from subterranean organs (S) of Nephrolepis cordifolia (NC) and Nephrolepis exaltata (NE) (F. Lomariopsidaceae) grown in Egypt was carried out. Gas Chromatography/mass Spectrometry (GC/MS) analysis of the hydro-distilled NCS and NES oils revealed that oxygenated compounds were abundant, being 55.35% and 33.85%, respectively. Ethyl ...

A reliable, relatively simple method for isolation and quantification of disaturated lecithins is described. In rabbit lung, 34% of the lecithins were disaturated, in alveolar macrophages, 19%. More than 95% of the fatty acids of the disaturated lecithins from lung and alveolar macrophages was palmitic. Hence, the disaturated lecithins from these sources were essentially all dipalmitoyl lecithi...

The present study aimed to investigate the essential oil composition of Liparis nervosa (Thunb.) Lindl., grown in China, and determine its antioxidant inhibitory effects on acetylcholinesterase. was obtained by hydrodistillation, chemical compounds were analyzed GC-MS GC-FID. We used 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric redu...

Latent heat storage (LHS) represents a valuable technology for the integration of intermittent renewable energy sources in existing and future systems. Improvements LHS can be sought by enhancing transfer efficiency, compactness diminishing environmental impact In this paper, direct contact latent (DC-LHS) using esters as phase change material (PCM) is proposed promising compact to achieve high...

For simple removal of saturated fatty acid methyl esters (FAMEs) from a FAME mixture, our previous research described winterization using sorbitan palmitate without agitation. However, limited information exists on additive real oil biodiesels and their separation performance. We demonstrated the biodiesel fuel derived commercial eatable oils fat (palm, lard, cottonseed, rice, soybean). Five fu...

AIM To determine the role of G0/G1 switch gene 2 (G0S2) and its transcriptional regulation in palmitate-induced hepatic lipid accumulation. METHODS HepG2 cells were treated with palmitate, or palmitate in combination with CCAAT/enhancer binding protein (C/EBP)β siRNA or G0S2 siRNA. The mRNA expression of C/EBPβ, peroxisome proliferator-activated receptor (PPAR)γ and PPARγ target genes (G0S2, ...

Endothelial cells lining the blood vessels are principal players in vascular inflammatory responses. Dysregulation of endothelial cell function caused by hyperglycemia, dyslipidemia, and hyperinsulinemia often result in impaired vasoregulation, oxidative stress, inflammation, and altered barrier function. Various stressors including high glucose stimulate the release of nucleotides thus initiat...