background: erythrocyte sedimentation rate (esr) is one of the predictors of improvement in handling rheumatoid arthritis. this study was designed to define and compare the time of achieving normal esr and also the percentage for the normalization of this marker at several points of time in two different combination therapies.methods: fifty-two rheumatoid arthritis patients randomly received me...

Our earlier studies showed that both carrier-mediated influx and efflux of [3H]methotrexate in murine tumor cells was in hibited by probenecid. The concentration of probenecid re quired for inhibition of influx was 10-fold greater than that required to inhibit efflux. As a consequence, the intracellular exchangeable level of [3H]methotrexate at steady state was markedly increased. Since these e...

The synthesis and retention of methotrexate polyglutamate derivatives were evaluated in the Ehrlich ascites tumor cell and the intestinal mucosa in vivo. When tumor-bearing mice re ceived injections of methotrexate (12 mg/kg), polyglutamate derivatives accumulated within the tumor cells as long as intracellular methotrexate was above the dihydrofolate reductase-binding capacity, the metabolites...

Background and aims: A selective and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been established for determination of methotrexate in human plasma. Methods: Methotrexate was extracted from plasma with acetonitrile. The mobile phase consisted of acetonitrile-water-formic acid 74: 25: 1(v/v). Twenty...

The antitumor effects of methotrexate with 5-fluorouracil against the Sarcoma 180 mouse tumor model were found to be schedule dependent. Pretreatment of tumor-bearing animals with methotrexate significantly enhanced the antitumor activity of the combination relative to simultaneous treatment or to those with methotrexate following 5-fluorouracil. These studies indirectly support the hypothesis ...

We report an enzyme immunoassay procedure for methotrexate measurement that takes less than 3 h to perform. beta-D-Galactosidase (EC 3.2.1.23) from Escherichia coli was conjugated to methotrexate by means of the mixed anhydride reaction. Bound and free labeled drug were separated by a preincubated cubic complex of first and second antibody. The enzyme activity of the bound fraction was measured...

REFERENCES 1. Buchbinder R, Hall S, Sambrook PN et al. Methotrexate therapy in rheumatoid arthritis; A life table review of 587 patients treated in community practice. / Rheumatol 1993,2fc639-44. 2. Segal R, Yaron M, Tartakovsky B. Methotrexate: mechanism of action. Semin Arthritis Rheum 1990-.20: 190-200. 3. Cronstein BN, Naime D, Ostad E. The anti-inflammatory mechanism of low dose methotrexa...

Methotrexate has been widely used for many years in the treatment of a variety of diseases. Acute pneumonitis and bone marrow suppression are very serious side effects in methotrexate treatment. A 48-year-old man with end-stage renal disease undergoing chronic hemodialysis developed combined acute pneumonitis and pancytopenia after a cumulative dose of 20 mg methotrexate for bullous pemphigoid....

Ectopic pregnancy (EP) refers to blastocyst implantation outside the uterine endometrium. EP is major cause of maternal morbidity and mortality. Treatment options include surgery, medical therapy with methotrexate, or expectant management. Methotrexate primary regimen used in cases early, unruptured ectopic pregnancies. Most side effects methotrexate are minor, including nausea, vomiting, abdom...

Results Of 125 patients assessed, 38 patients refused consent. Of 76 eligible patients, 23 were enrolled from the previous randomised trial and 53 from an open label study. At 40 weeks, 65% (26/40) of methotrexate treated patients remained in remission compared with 39% (14/36) of those receiving placebo injections (p=0.04, absolute reduction in risk of relapse 26%, 95% confidence intervals 4–4...