BACKGROUND Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein production is under the control of the strong methanol-inducible aox1 promoter. However, detailed information about the physiological alterations in P. pastoris accompanying the shift from growth on glycerol to methanol-induced protein production under industrial relevant...

Background Formic acid (formate) is the main reason for toxicity and death through methanol poisoning. The simultaneous determination of methanol, ethanol, and formate in the body can help to discover the cause of death and is useful in the diagnosis of acute methanol poisoning. The measurement of formate is not yet available in Iran. With regard to the increasing rate of methanol poisoning and...

We recently showed that methanol emitted by wounded plants might function as a signaling molecule for plant-to-plant and plant-to-animal communications. In mammals, methanol is considered a poison because the enzyme alcohol dehydrogenase (ADH) converts methanol into toxic formaldehyde. However, the detection of methanol in the blood and exhaled air of healthy volunteers suggests that methanol m...

Methanol is an abundant atmospheric volatile organic compound that is released from both living and decaying plant material. In forest and other aerated soils, methanol can be consumed by methanol-utilizing microorganisms that constitute a known terrestrial sink. However, the environmental factors that drive the biodiversity of such methanol-utilizers have been hardly resolved. Soil-derived iso...

We present interferometric observations with∼3.5′′ resolution of the 2k → 1k methanol lines towards the methanol masers in the DR21/DR21(OH) complex. These transitions are not masing and hence our measurements can be used to place limits on the methanol mass associated with the maser spots. We do not find in general good correlation between the peaks of “non-maser” methanol emission and the pos...

A 22-year-old male presented with acute onset vomiting, diarrhea, abdominal pain and altered sensorium. He had a history of acute alcohol intake (locally made with solvent) a day prior to the presentation. Biochemical investigations revealed severe metabolic acidosis. Diagnosis of methanol poisoning was made on the basis of history and biochemical abnormality. Magnetic resonance imaging (MRI) d...

a sodium dodecyl sulfate modified carbon paste electrode (sds/cpe) was developed in this work based on the surface modification method. the modified electrode was prepared by immersing sds/cpe in a nickel nitrate solution. this electrode showed a strong accumulation ability toward nickel ions. then modified electrode was conditioned by potential recycling in a potential range of 0.2–0.8 v (vs. ...

feasibility of methanolic wastes treatment containing chlorophenol in experimental uasb reactors i.e. r-i and r-ii working side by side constantly for a couple of almost 32 weeks were studied. uasb reactor r-i was initiated by utilizing the digested sludge alone, whereas, the uasb reactor r-ii was startedup by adding an activated carbon of an effective size 1.5-2.5mm to the digested sludge with...