Four in vitro experiments (Exp.) were conducted to evaluate lavender essential oil (LEO) effects at 0 (control), 250 (low dose), 500 (medium dose), 750 and 1000 µL per L(high doses) of incubation medium on rumen gas production kinetics (Exp.1), ruminal digestibility and fermentation (Exp.2), methane production (Exp.3) and rumen acidosis (Exp.4). The asymptote of gas production (A) incr...

This study using the rumen simulation technique (RUSITEC) investigated the changes in the ruminal microbiota and anaerobic fermentation in response to the addition of different lipid supplements to a ruminant diet. A basal diet with no oil added was the control, and the treatment diets were supplemented with sunflower oil (2%) only, or sunflower oil (2%) in combination with fish oil (1%) or alg...

Alpine forages are assumed to have specific effects on ruminal digestion when fed to cattle. These effects were investigated in an experiment from two perspectives, either by using such forages as a substrate for incubation or as feed for a rumen fluid donor cow. In total, six 24-h in vitro batch culture runs were performed. Rumen fluid was collected from a non-lactating donor cow after having ...

Three commercial plant tannin and a yucca extract having different secondary compound profiles were simultaneously evaluated in vitro for their effects on ruminal methane gas production and rumen fermentation patterns of wheat forage. Overall objective was to quantify in vitro, the sources of tannins and dose levels of tannins and yucca extract on rate of gas production, ruminal fermentation, f...

The fermentation process in which organic material is degraded and biogas (composed of mainly methane and carbon dioxide) is produced, is referred to as anaerobic digestion. Anaerobic digestion processes occur in many places where organic material is available and redox potential is low (zero oxygen). This is typically the case in stomachs of ruminants, in marshes, sediments of lakes and ditche...

Stadtman and Barker (1949) using C14 labeled C02 demonstrated that the methane produced in this fermentation is formed entirely by reduction of C02. Experiments with growing cultures led to the conclusion (Barker, 1939, 1956) that the oxidation of alcohol is dependent upon the supply of carbon dioxide, and that when the carbon dioxide is consumed, no further oxidation of alcohol occurs. This pa...

Background Cashew nut shell liquid (CNSL) is an agricultural byproduct containing alkylphenols that has been shown to favorably change the rumen fermentation pattern only under experimentally fixed feeding conditions. Investigation of CNSL potency in rumen modulation under a variety of feeding regimens, and evidence leading to the understanding of CNSL action are obviously necessary for further...

The estimated diffusion fluxes of methane (CH4) and carbon dioxide (CO2) at the sediment-water interface in the Rzeszów Reservoir in southeastern Poland are presented. The relevant studies were conducted during 2009, 2010, and 2011. Calculated fluxes ranged from 0.01 to 2.19 mmol m-2 day-1 and from 0.36 to 45.33 mmol m-2 day-1 for methane and carbon dioxide, respectively. While the values for c...

The objective of this work was to study nutritional strategies for decreasing methane production by ruminants fed tropical diets, combining in vitro and in vivo methods. The in vitro approach was used to evaluate the dose effect of condensed tannins (CT) contained in leaves of Gliricidia sepium, Leucaena leucocephala, and Manihot esculenta (39, 75, and 92 g CT/kg DM, respectively) on methane pr...

Plant saponins have been used as additives to mitigate methane emissions originated from enteric fermentation of ruminant animals. Most of the studies however were based on in vitro techniques, and only limited studies were directly conducted on the animals. Inconsistencies of saponin effects on methane emissions have been previously observed, which might be related to their levels in rations. ...