BACKGROUND Short half-life and low levels of growth factors in the niche of injured microenvironment necessitates the exogenous and sustainable delivery of growth factors along with stem cells to augment the regeneration of injured tissues. METHODS Here, recombinant human hepatocyte growth factor (HGF) was incorporated into chitosan nanoparticles (CNP) by ionic gelation method and studied for...

BACKGROUND/AIMS Hematopoiesis can occur in the liver, when the bone marrow fails to provide an adequate environment for hematopoietic stem cells. Hepatic stellate cells possess characteristics of stem/progenitor cells, but their contribution to hematopoiesis is not known thus far. METHODS Isolated hepatic stellate cells from rats were characterized with respect to molecular markers of bone ma...

Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then different...

Abstract Stem cells have been introduced as new promising therapeutic agents in treatment of degenerative diseases because having high differentiation potential while maintaining the ability to self-replicate and retaining features their source cells. Among different type cell therapies, mesenchymal stromal/stem (MSC) therapy is being increasingly developed a way treat structural defects that n...

PURPOSE Limbal stem cell deficiency is a challenging clinical problem and the current treatment involves replenishing the depleted limbal stem cell (LSC) pool by either limbal tissue transplantation or use of cultivated limbal epithelial cells (LEC). Our experience of cultivating the LEC on denuded human amniotic membrane using a feeder cell free method, led to identification of mesenchymal cel...

AIMS The heart contains c-kit(+) progenitors that maintain cardiac homeostasis. Cardiac c-kit(+) cells are multipotent and give rise to myocardial, endothelial and smooth muscle cells, both in vitro and in vivo. C-kit(+) cells have been thoroughly investigated for their stem cell activity, susceptibility to stress conditions and ageing, as well as for their ability to repair the infarcted heart...

background: pancreatic islet transplantation has emerged as a promising treatment for type i diabetes. however, its efficacy is severely hampered due to poor islet engraftment and revascularization, which have been resulted to partially loss of transplanted islets. it has been shown that local delivery of vascular endothelial growth factor (vegf) could accelerate transplanted islet revasculariz...

Exosomes are nanosized membrane-bound extracellular vesicles with the capacity to modify functions and behaviors of recipient cells via transferring unique biological information. Mounting evidence suggests that under developmental conditions, mesenchymal cell-derived exosomes capable mediating intercellular communication between epithelium mesenchyme during organogenesis salivary gland, tooth,...

Continued improvements in the understanding and application of mesenchymal stem cells (MSC) have revolutionized tissue engineering. This is particularly true within the field of skeletal regenerative medicine. However, much remains unknown regarding the native origins of MSC, the relative advantages of different MSC populations for bone regeneration, and even the biologic safety of such unpurif...