Three murine hybridomas (TMMR-1-3) were developed by repeated immunizations of mice with four different human osteosarcoma cell lines in an alternating sequence of inoculations. The monoclonal antibodies were screened for reactivities to cultured cell lines and tissue sections of osteosarcomas using flow cytometry and immunohistochemical techniques. TMMR-2 is a highly specific antibody (IgG1) t...

Bovine pancreatic deoxyribonuclease A was selectively cleaved by digestion with a-chymotrypsin under carefully controlled conditions. The peptide bond in DNase A between tryptophan residue 178 and serine 179 was quantitatively hydrolyzed in less than 15 min by 0.5 % ac-chymotrypsin at pH 8.5 and 4” when divalent metal ions were absent. The modified DNase A (DNase A (l-178,179-257)) was composed...

Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for human IgG, IgA, or IgM in the IFA procedure. The effects of heat and mercaptoethan...

The wheat seed used was stored at 4 f 1 "C until required and before use was washed with approx. 2 litres of distilled water per lOOg of seed. The seeds were germinated in the dark on moist filter papers a t 20"C+ 1°C. The extraction of chromatin from the ungerminated seeds was based on that of Simon & Becker (1976). Approx. 25g of dry seed was washed, then homogenized in approx. lOOml of extra...

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stag...

The effects of transglutaminase (TGase), reductant, and thermal treatment on the cross-linking white proteins in soft-shell turtle eggs were investigated. Egg denatured by reductant (0.83% 2-mercaptoethanol, 2-ME) pretreatment (95 °C 5 min), then catalyzed TGase (1.0 unit/mL). SDS–PAGE showed that without any pretreatments, three major egg (210 kDa, 115 76 kDa proteins) inferior substrates for ...