We have proposed the neck linker swing model to investigate the mechanism of mechanochemical coupling of kinesin. The Michaelis-Menten-like curve for velocity vs ATP concentration at different loads has been obtained, which is in agreement with experiments. We have predicted that Michaelis constant doesn’t increase monotonically and an elastic instability will happen with increasing of applied ...

Generation and inhibition of activated factor IXa was studied in factor XIa-activated plasma containing 4 mmol/L free calcium ions and 20 mumol/L phospholipid (25 mol% phosphatidylserine/75 mol% phosphatidylcholine). Interference of other (activated) clotting factors with the factor IXa activity measurements could be avoided by using a highly specific and sensitive bioassay. Factor IXa generati...

Application of Fick's ®rst law to substrate undergoing chemical reaction catalyzed by an enzyme immobilized in a porous slab-shaped bead leads to substrate concentration pro®les that are ̄atter when the ratio of stoichiometric coef®cients of product to reactant (m) is lower. Since the actual effectiveness factor decreases when m increases (at approximately the same rate irrespective of the valu...

The DynaFit software package (http://www.biokin.com/dynafit/) contains a builtin (“hard-coded”) implementation of the integrated Michaelis-Menten equation formulated in terms of the Lambert omega function. This built-in regression model can be used conveniently to determine the substrate kinetic parameters either from a single progress curve or from a collection of enzymatic progress curves ana...

in this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. study of  the stability of the resulted sulfite biosensor revealed that formation of a passive fil...

The specificity of an enzyme obeying the Michaelis−Menten equation is normally measured by comparing the kcat/Km for different substrates, but this is inappropriate for enzymes with a Hill coefficient h different from 1. The obvious alternative of generalizing Km in the expression as K0.5, the substrate concentration for half-saturation, is better, but it is not entirely satisfactory either, an...

The effects of various metal ions (Na, K, Ca, Mg, Al, Co) and anions (Cl, and CH3COO) on two cellulases were investigated. Fitting of the data to Michaelis-Menten kinetics showed that Al noncompetitively inhibited cellulase (Km = 22.68 g/L; Vmax = 0.269 mg/min at 5.0 mmol/L AlCl3) and Mg competitively inhibited cellulase (Km = 50.0 g/L; Vmax = 0.434 mg/min at 10.0 mmol/L MgCl2) Different metal ...

1. A polarographic method of measuring and continuously recording the concentration of oxygen in solution down to the order of 1O-9M has been described. 2. This method has been used to determine the respiration rate of bacteria as a function of oxygen concentration. 3. The results so obtained have shown that the respiration rate of bacteria is related to the oxygen concentration by the Michaeli...