نتایج جستجو برای: membrane stabilizing
تعداد نتایج: 409810 فیلتر نتایج به سال:
aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. elisa is one of the sensitive methods for detection of aflatoxins. preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an elisa method. in current study, different stabilizers were applied to stabilize a newly prepared con...
Dynamin is a master regulator of membrane fission in endocytosis. However, a function for dynamin immediately upon fusion has also been suspected from a variety of experiments that measured release of granule contents. The role of dynamin guanosine triphosphate hydrolase (GTPase) activity in controlling fusion pore expansion and postfusion granule membrane topology was investigated using polari...
Inflammation is a normal protective response to tissue injury caused by physical trauma, noxious chemical or microbial agents. The lysosomal enzymes released during inflammation produced a variety of disorders. The extracellular activity of these enzymes is said to be related to acute or chronic inflammation. Stabilization of lysosomal membrane is important in limiting the inflammatory response...
The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37 degrees C in Ringer's solutions containing from 0.1 to 100.0 microg/ml of cationic polymers, and subsequently was fixed with 1% OsO(4) and examined with the elec...
this is a comprehensive review of the recent progresses made in the field of zeolite membranes. it describes zeolitic materials and methods of membrane fabrication, followed by a summary of applications for gas separation, pervaporation and separation of liquid mixtures. special attention is called to polymer mixed matrix membranes (mmms) and membranes based on metal organic frame works (mofs)....
Membrane recruitment of coatomer and formation of coat protein I (COPI)-coated vesicles is crucial to homeostasis in the early secretory pathway. The conformational dynamics of COPI during cargo capture and vesicle formation is incompletely understood. By scanning the length of δ-COP via functional complementation in yeast, we dissect the domains of the δ-COP subunit. We show that the μ-homolog...
PICK1 is a neuronal scaffolding protein containing a PDZ domain and an auto-inhibited BAR domain. BAR domains are membrane-sculpting protein modules generating membrane curvature and promoting membrane fission. Previous data suggest that BAR domains are organized in lattice-like arrangements when stabilizing membranes but little is known about structural organization of BAR domains in solution....
There is a compelling and growing need to accurately predict the impact of amino acid mutations on protein stability for problems in personalized medicine and other applications. Here the ability of 10 computational tools to accurately predict mutation-induced perturbation of folding stability (ΔΔG) for membrane proteins of known structure was assessed. All methods for predicting ΔΔG values per...
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