phytic acid is the dominant form of phosphorous in plant seeds. however, phytic acid cannot beutilized by animals and causes them serious phosphate deficiency. utilization of phytase as ananimal feed additive can affect liberation of phosphor and its mineral availability. in this study,heterologous expression of the a. niger phya gene was investigated in the methylotrophic yeastsp. pastoris and...

Xylaria, belonging to the Ascomycotina, is known to produce diverse classes of bioactive substances. In an effort to identify the chemical constituents of the fruiting bodies of Xylaria polymorpha, linoleic acid (1), linoleic acid methyl ester (2), ergosterol (3), 4-acetyl-3,4-dihydro-6,8-dihydroxy-3-methoxy-5-methyl-1H-2-benzopyran-1-one (4), and 4-hydroxyscytalone (5) were isolated from its m...

background and objectives: during past decades hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer. materials and methods: the corresponding nucleotide sequences for the expression of heterologous genes in hansenula poylmorpha were extracted and as...

The first record of the quagga mussel Dreissena rostriformis bugensis (Andrusov, 1897) in Germany is described. This species has expanded its distribution area in Europe at a slower rate than the zebra mussel Dreissena polymorpha (Pallas, 1771). Recent records from the Danube in Romania and from an enclosed Rhine-Meuse estuary in The Netherlands, suggest that the MainDanube canal and River Rhin...

A 47 kDa integral peroxisomal membrane protein (PMP47) of Candida boidinii was expressed in wild type (WT) and a temperature-sensitive (Ts6) peroxisome-deficient (per) mutant of Hansenula polymorpha. The subcellular location of PMP47 appeared to be dependent on the level of expression. At low expression levels PMP47 was sorted to the peroxisomal membrane; however, in Ts6 cells grown at restrict...

Simple sequence repeat (SSR) or microsatellite DNA markers have been shown to function well in plant and mammalian species for genetic map construction and genotype identification. The objectives of the work reported here were to search GenBank for the presence of SSR-containing sequences from the genus Medicago, to assess the presence and frequency of SSR DNA in the alfalfa (Medicago sativa (L...