BACKGROUND AND OBJECTIVE Platelet-rich plasma is used to deliver growth factors, in a safe and convenient manner, for enhancing bone and periodontal regeneration. However, conflicting reports regarding its effectiveness suggest that further study of the relevant cellular mechanisms is required. The aim of this study was to investigate the in vitro effect of platelet-rich plasma on osteoblasts a...

Periodontitis is a widespread dental disease affecting 10 to 15% of worldwide adult population, yet the current treatments are far from satisfactory. The human periodontal ligament stem cell is a promising potential seed cell population type in cell-based therapy and tissue regeneration, which require appropriate scaffold to provide a mimic extracellular matrix. Zein, a native protein derived f...

During orthodontic tooth movement, the periodontal ligament (PDL) is exposed to continuous mechanical strain. However, many researchers have applied cyclic tensile strain, not continuous tensile strain, to PDL cells in vitro because there has been no adequate device to apply continuous tensile strain to cultured cells. In this study, we contrived a novel device designed to apply continuous tens...

Enamel matrix derivative (EMD), a porcine extract harvested from developing porcine teeth, has been shown to promote formation of new cementum, periodontal ligament and alveolar bone. Despite its widespread use, an incredibly large variability among in vitro studies has been observed. The aim of the present study was to determine the influence of EMD on cells at different maturation stages of o...

In order to provide the best long-term prognosis for a tooth that is to be replanted intentionally, the tooth must be kept out of the socket for the shortest period possible [4], and the extraction of the tooth should be atraumatic to minimise damage to the cementum and the periodontal ligament [5]. The periodontal ligament attached to the root surface should be kept moist in saline, Hank’s Buf...

This paper presents the development of a novel application using motion analysis techniques to measure tooth movement in 3D. Measuring the relative displacements of teeth under load allows a greater understanding of the mechanical properties of the periodontal ligament (PDL). Two marker clusters were fixed onto the measured tooth and the reference tooth via orthodontic brackets and movements we...

Background: Stem cells have been widely used in various fields of the health sector, one which is dental health. Teeth with malocclusion require orthodontic treatment to achieve good function and aesthetics. Orthodontic tooth movement (OTM) occurs due a bone remodeling process, namely deposition areas tension resorption pressure. Differentiated stem are thought influence OTM through several dif...

Peripheral ossifying fibroma is a common solitary gingival overgrowth thought to arise from the gingival corium, periosteum or periodontal ligament. Ossifying fibroma has the propensity to occur in cranial bones, but the peripheral variety shows a close relationship with the periodontal ligament and adjacent alveolar bone. It is more commonly seen in women (predilection of 3:1) and usually at a...

Human periodontal ligament cells (hPDLCs), with the potential for multi-directional differentiation and reproduction, are the target cells of orthodontic tooth movement. The aim of this study was to examine the effect of mechanical tension force and lipopolysaccharides (LPS) on hPDLCs and whether they induce proliferative and differentiated characters in vitro. Tension force was applied to hPDL...