The present study aims at the phytochemical profiling of lyophilized pure fruit juice powder of Citrus limon and demonstrates its efficacy as an antitumor agent against human breast cancer cell line (MCF-7). The cytotoxicity was evaluated using the changes in cell morphology, cell viability, and DNA fragmentation, the percentage of cell viability was determined by MTT assay. Our results showed ...

In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include hum...

A new series of anti-cancer agents based on 1,2-diaryl-5,6-dihydropyrrolo[2,1-a]isoquinoline scaffold containing N,N-diethylamino‌ethoxy, piperidinyl‌ethoxy or morpholinyl‌ethoxy group at the para position of the C-2 phenyl ring were synthesized and their cytotoxic activities were assessed against several human cancer cell lines including MCF-7 (ER positive breast cancer cell), MDA-MB231 (ER-ne...

Cytotoxic activity of gaillardin, a sesquiterpene lactone isolated from Inula oculus-christi L. (Asteraceae), was assessed in the human breast adenocarcinoma cell line MCF-7, human hepatocellular carcinoma cell line HepG-2, human non-small cell lung carcinoma cell line A-549, and human colon adenocarcinoma cell line HT-29, resulting in IC50 values of 6.37, 6.20, 4.76, and 1.81 microg/mL, respec...

Preclinical investigations have identified insulin-like growth factor (IGF) signaling as a key mechanism for cancer growth and resistance to clinically useful therapies in multiple tumor types including breast cancer. Thus, agents targeting and blocking IGF signaling have promise in the treatment of solid tumors. To identify possible mechanisms of resistance to blocking the IGF pathway, we gene...

conclusions the increasing cell toxicity, apoptosis-inducing effects and decreasing telomerase activity may play an important role in the valproic acid and radiation mechanism. the current survey suggested that it is likely beneficial to combine valproic acid and gamma radiation to treat breast cancer. results combination of valproic acid and gamma radiation increased significantly cell toxicit...

conclusions these outcomes support our theory that rapamycin increases oxidative stress in mcf-7 and mda mb-231 cells but also shows high levels of antioxidant effects, which probably limit the effects of the rapamycin on the same issue in the clinic. methods the mcf-7 and mda mb-231 cell lines were cultivated and treated with rapamycin for 72 hours. the viability of the cells was determined us...

Objective(s):According to the prevalence of neoplastic diseases, there is a deep necessity for discovery of novel anticancer drugs in the field of medicinal chemistry. In the current study, a new series ofphenylthiazole derivatives(compounds 4a-4f) was synthesizedand theiranticancer activity was assessed in vitro. Materials and Methods:All synthesized derivatives were evaluated towards three h...