Commerical interest in the acetone-butanol fermentation has prompted considerable investigation into methods of solvent production. The bulk of the work on the subject, however, has been concerned chiefly with methods of increasing solvent yields, whereas relatively few studies have dealt with the intermediary metabolism involved. Johnson, Peterson, and Fred (1933) first demonstrated clearly th...

Fatty acids can be synthesized in mitochondrial membranes of rat liver either by elongation or by synthesis de nouo. The outer membranes incorporate greater amounts of substrate into fatty acids than do the inner membranes. Outer membranes synthesize 6and g-carbon fatty acids completely de nouo, while smaller amounts of lo-carbon acids are produced by the mechanism de novo. The reactions de nov...

Fatty acids can be synthesized in mitochondrial membranes of rat liver either by elongation or by synthesis de nouo. The outer membranes incorporate greater amounts of substrate into fatty acids than do the inner membranes. Outer membranes synthesize 6and g-carbon fatty acids completely de nouo, while smaller amounts of lo-carbon acids are produced by the mechanism de novo. The reactions de nov...

The fatty acid-synthesizing system of Escherichia coli w fractionated by chromatography on diethylaminoethyl cellulose into three different enzyme fractions: Err, ErrI, a E rv. Incubation of Fraction Err with acetyl coenzyme malonyl coenzyme A, reduced triphosphopyridine nucleotide, and acyl carrier protein (ACP) yielded /GhydroxydecanoylACP, ,l3-hydroxylauryl-ACP, and /3-hydroxymyristyl-ACP va...

Biosynthesis of very long chain (>C(18)) fatty acids (VLCFAs) and the pathway for their incorporation into acyl lipids was studied in microspore-derived (MD) and zygotic embryos of Brassica napus L. cv Reston. In the presence of [1-(14)C]oleoyl-coenzyme A or [1-(14)C] eicosenoyl-coenzyme A, malonyl-coenzyme A, and reducing equivalents, maximal in vitro elongation activity was expressed in prote...

MCD (malonyl-CoA decarboxylase), which catalyses decarboxylation of malonyl-CoA, is known to play an important role in the regulation of malonyl-CoA concentration. Recently, it has been observed that the expression of MCD is significantly decreased in the hearts of the PPARalpha (peroxisome-proliferator-activated receptor alpha) (-/-) mice, where the rate of fatty-acid oxidation is decreased by...